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BCL-10 Antibody

Purified Mouse Monoclonal Antibody

     
  • WB - BCL-10 Antibody AO1003a
    Figure 1: Western blot analysis using BCL10 mouse mAb against NIH/3T3 (1), Hela (2), MCF-7 (3) and Jurkat (4) cell lysate.
    detail
  • IHC - BCL-10 Antibody AO1003a
    Figure 2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma (A) and liver carcinoma (B), showing cytoplasmic localization using BCL10 mouse mAb with DAB staining.
    detail
  • IF - BCL-10 Antibody AO1003a
    Figure 3: Confocal immunofluorescence analysis of Hela cells using BCL10 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
    detail
  • FC - BCL-10 Antibody AO1003a
    Figure 4: Flow cytometric analysis of Hela cells using BCL10 mouse mAb (green) and negative control (purple).
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, FC, ICC, E
Primary Accession O95999
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Clone Names 4F8
Isotype IgG1
Calculated MW 26kDa
Description Bcl-10 (B-cell CLL/lymphoma 10), also known as CLAP, Me10, CIPER, c-E10, CARMEN. Entrez Protein NP_003912. It is a protein containing a caspase recruitment domain (CARD). It plays an important role in apoptosis and activating NF-kappaB. The research suggested that it interacted with other CARD domain containing proteins including CARD9, 10, 11 and 14, which were thought to function as upstream regulators in NF-kappaB signaling. Bcl-10 is found to form a complex with MALT1 which encoded by another gene known to be translocated in MALT lymphoma. MALT1 and Bcl-10 are thought to synergize in the activation of NF-kappaB, and the deregulation of either of them may contribute to the same pathogenetic process that leads to the malignancy.
Immunogen Purified recombinant fragment of human BCL-10 expressed in E. Coli.
Formulation Purified antibody in PBS containing 0.03% sodium azide.
Additional Information
Gene ID 8915
Other Names B-cell lymphoma/leukemia 10, B-cell CLL/lymphoma 10, Bcl-10, CARD-containing molecule enhancing NF-kappa-B, CARD-like apoptotic protein, hCLAP, CED-3/ICH-1 prodomain homologous E10-like regulator, CIPER, Cellular homolog of vCARMEN, cCARMEN, Cellular-E10, c-E10, Mammalian CARD-containing adapter molecule E10, mE10, BCL10, CIPER, CLAP
Dilution WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBCL-10 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BCL10 {ECO:0000303|PubMed:9989495, ECO:0000312|HGNC:HGNC:989}
Function Plays a key role in both adaptive and innate immune signaling by bridging CARD domain-containing proteins to immune activation (PubMed:10187770, PubMed:10364242, PubMed:10400625, PubMed:24074955, PubMed:25365219). Acts by channeling adaptive and innate immune signaling downstream of CARD domain-containing proteins CARD9, CARD11 and CARD14 to activate NF-kappa-B and MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) pathways which stimulate expression of genes encoding pro-inflammatory cytokines and chemokines (PubMed:24074955). Recruited by activated CARD domain-containing proteins: homooligomerized CARD domain-containing proteins form a nucleating helical template that recruits BCL10 via CARD-CARD interaction, thereby promoting polymerization of BCL10, subsequent recruitment of MALT1 and formation of a CBM complex (PubMed:24074955). This leads to activation of NF-kappa-B and MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) pathways which stimulate expression of genes encoding pro-inflammatory cytokines and chemokines (PubMed:18287044, PubMed:24074955, PubMed:27777308). Activated by CARD9 downstream of C-type lectin receptors; CARD9-mediated signals are essential for antifungal immunity (PubMed:26488816). Activated by CARD11 downstream of T-cell receptor (TCR) and B-cell receptor (BCR) (PubMed:18264101, PubMed:18287044, PubMed:24074955, PubMed:27777308). Promotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK (PubMed:10187815).
Cellular Location Cytoplasm, perinuclear region. Membrane raft. Note=Appears to have a perinuclear, compact and filamentous pattern of expression. Also found in the nucleus of several types of tumor cells. Colocalized with DPP4 in membrane rafts.
Tissue Location Ubiquitous..
Research Areas
Citations (0)
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References

1. Willis, T.G., et al. (1999) Cell. 96, 35-45. 2. Lucas, P.C., et al. (2001) J. Biol.Chem. 276, 19012-19019. 3. Wang, L., et al. (2001) J. Biol.Chem. 276, 21405-21409

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$ 300.00
Cat# AO1003a
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