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Anti-β-Catenin (Tyr-489) [γ-Catenin (Tyr-480)], Phosphospecific Antibody

     
  •  - Anti-β-Catenin (Tyr-489) [γ-Catenin (Tyr-480)], Phosphospecific Antibody AN1679
    Western blot analysis of A431 cells stimulated with pervanadate (1 mM) for 30 min (lanes 1, 3, & 5) then treated with akaline phosphatase (lanes 2, 4, & 6). The blot was probed with anti-γ-Catenin (CM1111), anti-β-Catenin (Tyr-489) conserved site (CP2961), or anti-β-Catenin (CM1181).
    detail
  •  - Anti-β-Catenin (Tyr-489) [γ-Catenin (Tyr-480)], Phosphospecific Antibody AN1679
    Immunocytochemical labeling of β-Catenin in pervanadate-treated A431 cells. The cells were labeled with mouse monoclonal β-Catenin (CM1181) or rabbit polyclonal β-Catenin (Tyr-489) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P35222
Reactivity Bovine
Host Rabbit
Clonality Rabbit Polyclonal
Isotype IgG
Calculated MW 85497 Da
Additional Information
Gene ID 1499
Other Names Catenin beta1, CTNNB1, catenin
Target/Specificity β-Catenin is a 92 kDa protein that binds to the cytoplasmic tail of E-Cadherin. The cadherins, transmembrane adhesion molecules, are found with catenins at adherens junctions. Deletions in the cytoplasmic domain of E-Cadherin eliminate catenin binding and result in a loss of cell adhesion. Tyrosine phosphorylation of β-Catenin can regulate its interaction with critical components of adherens junctions. Both Fer and Fyn kinases phosphorylate tyrosine 142 in vitro. Overexpression of these kinases in epithelial cells disrupts interactions between α- and β-Catenins. The phosphorylation of tyrosine 142 may act as a switch from the transcriptional to the adhesive role of β-Catenin. Src family kinases can also phosphorylate tyrosine 86 and 654 in β-Catenin. The Tyr-654 phosphorylation regulates β-Catenin binding to E-cadherin. Thus, site-specific tyrosine phosphorylation of β-Catenin may regulate protein-protein interactions leading to changes in cell adhesion.
Format Antigen Affinity Purified
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAnti-β-Catenin (Tyr-489) [γ-Catenin (Tyr-480)], Phosphospecific Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
ShippingBlue Ice
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Background

β-Catenin is a 92 kDa protein that binds to the cytoplasmic tail of E-Cadherin. The cadherins, transmembrane adhesion molecules, are found with catenins at adherens junctions. Deletions in the cytoplasmic domain of E-Cadherin eliminate catenin binding and result in a loss of cell adhesion. Tyrosine phosphorylation of β-Catenin can regulate its interaction with critical components of adherens junctions. Both Fer and Fyn kinases phosphorylate tyrosine 142 in vitro. Overexpression of these kinases in epithelial cells disrupts interactions between α- and β-Catenins. The phosphorylation of tyrosine 142 may act as a switch from the transcriptional to the adhesive role of β-Catenin. Src family kinases can also phosphorylate tyrosine 86 and 654 in β-Catenin. The Tyr-654 phosphorylation regulates β-Catenin binding to E-cadherin. Thus, site-specific tyrosine phosphorylation of β-Catenin may regulate protein-protein interactions leading to changes in cell adhesion.

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$ 329.00
Cat# AN1679
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