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Anti-Synaptotagmin (Thr202) Antibody

Our Anti-Synaptotagmin (Thr202) rabbit polyclonal phosphospecific primary antibody from PhosphoSolut

     
  •  - Anti-Synaptotagmin (Thr202) Antibody AN1570
    Immunostaining of 14 DIV rat cortical neurons labeling synaptotagmin when phosphorylated at Thr202 (cat. p1570-202, 1:400, red) and PSD95 (green). The cortical neurons were fixed with methanol at -20C for 10 minutes. Photo courtesy of Gang Liu.
    detail
  •  - Anti-Synaptotagmin (Thr202) Antibody AN1570
    Western blot of rat cortical lysate showing specific immunolabeling of the ~62 kDa synaptotagmin phosphorylated at Thr202 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P21707
Reactivity Bovine
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 47399 Da
Additional Information
Gene ID 25716
Other Names DKFZp781D2042 antibody, FLJ42519 antibody, P65 antibody, SVP65 antibody, synaptotagmin 1 antibody, Synaptotagmin I antibody, SYT antibody, SYT1 antibody, SytI antibody
Target/Specificity Synaptotagmin is widely regarded as the primary calcium sensor for synaptic vesicle exocytosis (Fernandez-Chacon et al., 2001; Wang et al., 2003). Moreover, recent studies indicate that the protein also plays a key role in endocytosis (Poskanzer et al., 2003). Synaptotagmin can be phosphorylated by multiple protein kinases and this may play a key role in modulation of synaptotagmin’s ability to influence both the exocytotic and endocytotic components of synaptic transmission (Hilfiker et al., 1999; Lee et al., 2004).
Format Antigen Affinity Purified from Pooled Serum
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAnti-Synaptotagmin (Thr202) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
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Background

Synaptotagmin is widely regarded as the primary calcium sensor for synaptic vesicle exocytosis (Fernandez-Chacon et al., 2001; Wang et al., 2003). Moreover, recent studies indicate that the protein also plays a key role in endocytosis (Poskanzer et al., 2003). Synaptotagmin can be phosphorylated by multiple protein kinases and this may play a key role in modulation of synaptotagmin’s ability to influence both the exocytotic and endocytotic components of synaptic transmission (Hilfiker et al., 1999; Lee et al., 2004).

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$ 430.00
Cat# AN1570
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