Synapsin I Antibody
Affinity purified rabbit polyclonal antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IF |
---|---|
Primary Accession | P17599 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | polyclonal |
Calculated MW | 78 KDa |
Gene ID | 281510 |
---|---|
Gene Name | SYN1 |
Other Names | Synapsin-1, Synapsin I, SYN1 |
Target/Specificity | Native protein purified from bovine brain. |
Dilution | WB~~ 1:1000 IF~~ 1:1000 |
Format | Prepared from rabbit serum by affinity purification using a column to which the native protein was coupled. |
Antibody Specificity | Specific for the ~78k synapsin I doublet in Western blots of rat brainextracts. Immunolabeling blocked by preadsorption of antibody with the protein used togenerate the antibody. |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | Synapsin I Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Shipping | Blue Ice |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002; Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. In addition to its role in plasticity, the expression of synapsin I is a precise indicator of synapse formation (Moore and Bernstein, 1989; Stone et al., 1994). Thus, synapsin I immunocytochemistry provides a valuable tool for the study of synaptogenesis. The role of synapsin in synaptic plasticity and in synaptogensis is regulated by phosphorylation (Jovanovic et al., 2001; Kao et al., 2002).
References
Feng J, Chi P, Blanpied TA, Xu YM, Magarinos AM, Fe
rreira A, Takahashi RH, Kao HT, McEwen BS, Ryan TA,
Augustine GJ, Greengard P (2002) Regulation of neurotransmitter release by synapsin III. J Neurosci 22:4372-
4380.
Jovanovic JN, Sihra TS, Nairn AC, Hemmings HC, Jr., Gr
eengard P, Czernik AJ (2001) Opposing changes in
phosphorylation of specific sites in synapsin I during Ca
2+
-dependent glutamate release in isolated nerve
terminals. J Neurosci 21:7944-7953.
Kao HT, Song HJ, Porton B, Ming GL, Hoh J, Abraham M,
Czernik AJ, Pieribone VA, Poo MM, Greengard P (2002) A
protein kinase A-dependent molecular switch in synapsin
s regulates neurite outgrowth. Nature Neurosci 5:431-
437.
Moore RY, Bernstein M (1989) Synaptogenesis in the rat suprachiasmatic nucleus demonstrated by electron
microscopy and synapsin I immunoreactivity. J Neurosci 9:2151-2162.
Nayak AS, Moore CI, Browning MD (1996) CaM kinase II
phosphorylation of the presyn
aptic protein synapsin is
persistently increased during expression of long-term po
tentiation. Proc Natl Acad Sci (USA) 93:15451-15456.
Stone LM, Browning MD, Finger TE (1994) Differential dist
ribution of the synapsins in the rat olfactory bulb. J
Neurosci 14:301-309.
Kurtis D. Davies, Susan M. Goebel-Goody, St
even J. Coultrap, and Michael D. Browning (2008)
Long Term Synaptic
Depression That Is Associated with GluR1 Dephosphorylation but Not
-Amino-3-hydroxy-5-methyl-4-
isoxazolepropionic Acid (AMPA) Receptor Internalization
J. Biol. Chem.,
283: 33138 - 33146.
Note: Dr. Michael Browning, co-author of the
cited papers is the President and founder
PhosphoSolutions.
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.