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Phospho-Ser234 Beclin-1 Antibody

Affinity purified rabbit polyclonal antibody

     
  • WB - Phospho-Ser234 Beclin-1 Antibody AN1225
    Western blot of flag-beclin-1 WT immunoprecipitates expressed in 293T cells showing specific immunolabeling of beclin-1 when phosphorylated at S234 (WT). This immunolabeling is decreased in the beclin-1 double alanine substitution mutant (S234A, S295A).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q14457
Reactivity Human
Predicted Bovine, Mouse, Monkey, Rat
Host Rabbit
Clonality polyclonal
Calculated MW 60 KDa
Additional Information
Gene ID 8678
Gene Name BECN1
Other Names Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197, BECN1, GT197
Target/Specificity Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser234 conjugated to KLH.
Dilution WB~~ 1:250
Format Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
Antibody Specificity Specific for the ~60k beclin1 protein phosphorylated at Ser234. Immunolabeling of the beclin-1 band is decreased in the beclin-1 alanine substitution mutant. Additional bands may be observed in non-immunoprecipitated lysates.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-Ser234 Beclin-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
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Research Areas
Citations (0)
citation

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Background

Beclin-1 plays a key role in tumor suppression and autophagy. It has been suggested that autophagy proteins such as beclin-1 can inhibit tumorigenesis and that decreased expression of these proteins may contribute to the development of human malignancies (Liang et al., 1999). Beclin-1 has been shown to interact with 14-3-3 proteins through Akt-mediated phosphorylation at Ser234 (Wang et al., 2012).

References

Liang, XH, Jackson S, Seaman M, Brown K, Kempkes B, Hibshoosh H, Levine B. (1999) Induction of autophagy and inhibition of tumorigenesis by beclin-1. Nature 402(6762):672-676.
Richard C. Wang, Yongjie Wei, Zhenyi An, Zhongju Zou, Guanghua Xiao, Govind Bhagat, Michael White, Julia Reichelt, and Beth Levine (2012) Akt-Mediated Regulation of Autophagy and Tumorigenesis Through Beclin 1 Phosphorylation. Science 338:956-959.

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$ 365.00
Cat# AN1225
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