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PEBP (Phosphatidylethanolamine Binding Protein) Antibody

Rabbit polyclonal antibody

     
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  • WB - PEBP (Phosphatidylethanolamine Binding Protein) Antibody AN1153
    All lanes : Anti-Pebp1 Antibody at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: C6 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: HepG2 whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 21 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • FC - PEBP (Phosphatidylethanolamine Binding Protein) Antibody AN1153
    Flow cytometric analysis of Hela cells using Park7 (DJ-1) Antibody(green, Cat#AN1153) compared to an isotype control of rabbit IgG(blue). AN1153 was diluted at 1:500 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC
Primary Accession P31044
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality polyclonal
Calculated MW 22 KDa
Additional Information
Gene ID 29542
Gene Name PEBP1
Other Names Phosphatidylethanolamine-binding protein 1, PEBP-1, 23 kDa morphine-binding protein, HCNPpp, P23K, Hippocampal cholinergic neurostimulating peptide, HCNP, Pebp1, Pbp, Pebp
Target/Specificity Purified native rat PEBP.
Dilution WB~~1:1000
FC~~1:500
Format Neat serum
Antibody Specificity Specific for the ~ 22k PEBP protein in Western blots
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPEBP (Phosphatidylethanolamine Binding Protein) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
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Research Areas
Citations (0)
citation

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Background

Phosphatidylethanolamine binding protein (PEBP), also known as Raf kinase inhibitory protein (RKIP) is a highly conserved multifunctional protein that modulates the Raf/MAPK signaling cascade and has been implicated as a suppressor of metastatic cancer (Trakul N et al., 2005). PEBP has been shown to not only inhibit cell proliferation but also to induce differentiation of human keratinocytes (Yamazaki et al., 2004). Dysregulation of calpain activity results in cellular degeneration in conditions such as Alzheimer’s disease and recently PEBP has been identified as a potential in vivo calpain substrate (Chen at al., 2006).

References

Trakul N, Menard RE, Schade GR, Qian Z, Rosner MR (2005) Raf kinase inhibitory protein regulates Raf-1 but not BRaf
kinase activation. J. Biol. Chem. 280(26): 24931-40.
Yamazaki T, Nakano H, Hayakari M, Tanaka M, Mayama J, Tsuchida S (2004) Differentiation induction of human
keratinocytes by phosphatidylethanolamine binding protein. J. Biol. Chem. 279(31):32191-95.
Chen Q, Wang S, Thompson SN, Hall ED, Guttmann RP (2006) Identification and characterization of PEBP as a
calpain substrate. J Neurochem. 99(4): 1133-41.

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$ 310.00
Cat# AN1153
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Availability: 7-10 days
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