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Neuron Specific Enolase (NSE) Antibody

Rabbit polyclonal antibody

     
  • WB - Neuron Specific Enolase (NSE) Antibody AN1152
    Western blot of rat cortex homogenate showing specific immunolabeling of the ~ 47kNSE protein.
    detail
  • IF - Neuron Specific Enolase (NSE) Antibody AN1152
    HEK 293 cells showing staining with anti-NSE antibody (red). The greenchannels shows staining for UCHL1.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IF
Primary Accession P09104
Reactivity Human, Rat
Host Rabbit
Clonality polyclonal
Calculated MW 47 KDa
Additional Information
Gene ID 2026
Gene Name ENO2
Other Names Gamma-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 2, Neural enolase, Neuron-specific enolase, NSE, ENO2
Target/Specificity Recombinant human NSE expressed in and purified from E. coli.
Dilution WB~~ 1:2000
IF~~ 1:500
Format Unpurified neat serum.
Antibody Specificity Specific for the ~47kDa NSE protein.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNeuron Specific Enolase (NSE) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
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Research Areas
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Background

Neuron specific enolase (NSE) is an enzyme which catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway, and also the reverse reaction in gluconeogenesis. It is one of three mammalian enolases, which are also known as ENO1, ENO2, and ENO3 or alternately as enolase alpha, beta and gamma. The three enolases have different cell type specific expression patterns, so that antibodies to them are useful cell type specific markers.(MacAlesse et al., 1988). NSE corresponds to ENO2 or enolase gamma and is heavily expressed in neuronal cells. ENO1 is also known as enolase alpha and as non-neuronal enolase. The third enolase, ENO3 or enolase beta, is expressed in muscle cells. Since neurons require a great deal of energy, they are very rich in glycolytic enzymes such a GAPDH and NSE. Antibodies to this protein are therefore useful to identify neuronal cell bodies, developing neuronal lineage and neuroendocrine cells. Release of NSE from damaged neurons into CSF and blood has also been used as a biomarker of neuronal injury (2).

References

1. MacAlesse SM, Dunbar B, Fotherqill JE, Hinks LJ, Day IN (1988) Complete amino acid
sequence of neurone-specific gamma isozyme of enolase (NSE) from human brain and
comparison with the non-neuronal alpha form (NNE). Eur J Biochem. 178(2):413-17.
2. Begaz, T., Kyriacou, D. N., Segal, J. and Bazarian, J. J. Serum biochemical markers for post-concussion syndrome in patients with mild traumatic brain injury. J. Neurotrauma 23:1201-1210 (2006).

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$ 310.00
Cat# AN1152
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