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NSF Antibody
Affinity purified rabbit polyclonal antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC, WB, FC |
|---|---|
| Primary Accession | Q9QUL6 |
| Reactivity | Rat |
| Predicted | Human, Mouse |
| Host | Rabbit |
| Clonality | polyclonal |
| Calculated MW | 75 KDa |
| Gene ID | 60355 |
|---|---|
| Gene Name | NSF |
| Other Names | Vesicle-fusing ATPase, N-ethylmaleimide-sensitive fusion protein, NEM-sensitive fusion protein, Vesicular-fusion protein NSF, Nsf, Erg1 |
| Target/Specificity | Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH. |
| Dilution | IHC~~1:500 FC~~1:500 WB~~ 1:1000 |
| Format | Prepared from rabbit serum by affinity purification via chromatography on an affinity column made with the C-terminal peptide used as antigen. |
| Antibody Specificity | Specific for ~75k NSF protein. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | NSF Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Shipping | Blue Ice |
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Provided below are standard protocols that you may find useful for product applications.
Background
NSF (N-ethylmaleimide sensitive fusion protein) is a critical component of the SNARE (soluble NSF attachment protein receptors) protein complex that is involved in synaptic vesicle trafficking. Specifically, NSF has been found to be essential in membrane fusion. Furthermore, NSF has been recently demonstrated to bind other protein complexes such as AMPA receptor subunits (GluR2), GATE-16, LMA-1 and Rabs suggesting a more diverse role in the assembly of various protein complexes (Whiteheart et al., 2004).
References
Schweizer FE, Dresbach T, DeBello WM, O’Connor V, Augustine G, Betz H (1998) Regulation of neurotransmitter release kinetics by NSF. Science 279(5354):1203-1206.
Matveeva EA, Whitehart SW, Vanaman T, Slevin J (2001) Phosphorylation of the N-ethylmaleimide sensitive factor is associated with depolarization-dependent neurotransmitter release from synaptosomes. J Biol.Chem. 276(15): 12174-12181.
Whitehart SW, Matveeva EA (2004) Multiple binding proteins suggest diverse functions for the N-ethylmaleimide sensitive factor. J Struct. Biol.146(1-2): 32-43.
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