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Tyrosine Hydroxylase Antibody

Affinity purified rabbit polyclonal antibody

     
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  • IHC - Tyrosine Hydroxylase Antibody AN1061
    Immunohistochemical analysis of paraffin-embedded R. brain section using Tyrosine Hydroxylase Antibody (Cat#AN1061). AN1061 was diluted at 1:1000 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
    detail
  • IHC - Tyrosine Hydroxylase Antibody AN1061
    Immunohistochemical analysis of paraffin-embedded M. brain section using Tyrosine Hydroxylase Antibody (Cat#AN1061). AN1061 was diluted at 1:1000 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
    detail
  • FC - Tyrosine Hydroxylase Antibody AN1061
    Flow cytometric analysis of PC-12 cells using Park7 (DJ-1) Antibody(green, Cat#AN1061) compared to an isotype control of rabbit IgG(blue). AN1061 was diluted at 1:1000 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
    detail
  • WB - Tyrosine Hydroxylase Antibody AN1061
    Western blot analysis of lysates from rat brain, mouse brain tissue lysate and U87-MG cell line(from left to right), using Th Antibody(Cat. #AN1061). AN1061 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
    detail
  • WB - Tyrosine Hydroxylase Antibody AN1061
    Western blot of 10 ug of rat caudate lysate showing specific immunolabeling of the ~60k TH protein.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
FC, WB, IHC
Primary Accession P04177
Reactivity Bovine, Human, Mouse, Rat
Host Rabbit
Clonality polyclonal
Calculated MW 60 KDa
Additional Information
Gene ID 25085
Gene Name TH
Other Names Tyrosine 3-monooxygenase, Tyrosine 3-hydroxylase, TH, Th
Target/Specificity SDS-denatured, native rat tyrosine hydroxylase purified from pheochromocytoma.
Dilution IHC~~1:1000
FC~~1:1000
WB~~ 1:1000
Format Prepared from rabbit serum by affinity purification using a protein A column and using a column to which the immunogen was coupled
Antibody Specificity Specific for the ~60k tyrosine hydroxylase protein.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTyrosine Hydroxylase Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
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Research Areas
Citations (0)
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Background

Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine and norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000; Salvatore et al., 2001; Dunkley et al., 2004).

References

Dunkley PR, Bobrovskaya L, Graham ME, Von Nagy-Felsobuki EI, Dickson PW (2004) Tyrosine hydroxylase phosphorylation: regulation and consequences. J Neurochem 91:1025-1043.
Kish SJ, Kalasinsky KS, Derkach P, Schmunk GA, Guttman M, Ang L, Adams V, Furukawa Y, Haycock JW (2001) Striatal dopaminergic and serotonergic markers in human heroin users. Neuropsychopharmacology 24:561-567.
Salvatore MF, Waymire JC, Haycock JW (2001) Depolarization-stimulated catecholamine biosynthesis: involvement of protein kinases and tyrosine hydroxylase phosphorylation sites in situ. J Neurochem 79:349-360.
Witkovsky P, Gabriel R, Haycock JW, Meller E (2000) Influence of light and neural circuitry on tyrosine hydroxylase phosphorylation in the rat retina. J Chem Neuroanat 19:105-116.
Xu ZQ, Lew JY, Harada K, Aman K, Goldstein M, Deutch A, Haycock JW, Hokfelt T (1998) Immunohistochemical studies on phosphorylation of tyrosine hydroxylase in central catecholamine neurons using site- and phosphorylation state-specific antibodies. Neurosci 82:727-738.
Zhu MY, Klimek V, Haycock JW, Ordway GA (2000) Quantitation of tyrosine hydroxylase protein in the locus coeruleus from postmortem human brain. J Neurosci Meth 99:37-44.
Zhu MY, Klimek V, Dilley GE, Haycock JW, Stockmeier C, Overholser JC, Meltzer HY, Ordway GA (1999) Elevated levels of tyrosine hydroxylase in the locus coeruleus in major depression. Biol Psychiatry 46:1275-1286.

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$ 365.00
Cat# AN1061
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Availability: 7-10 days
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