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RAB27B Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • WB - RAB27B Antibody AM8688b
    All lanes : Anti-RAB27B Antibody at 1:2000 dilution Lane 1: DU145 whole cell lysate Lane 2: mouse stomach lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • FC - RAB27B Antibody AM8688b
    Overlay histogram showing A431 cells stained with AM8688b(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AM8688b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession O00194
Reactivity Human, Mouse
Host Mouse
Clonality monoclonal
Isotype IgG1,k
Clone/Animal Names 1596CT245.254.49
Calculated MW 24608 Da
Additional Information
Gene ID 5874
Other Names Ras-related protein Rab-27B, C25KG, RAB27B
Target/Specificity This RAB27B antibody is generated from a mouse immunized with a recombinant protein of human RAB27B.
Dilution WB~~1:2000
FC~~1:25
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRAB27B Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RAB27B
Function Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate homeostasis of late endocytic pathway, including endosomal positioning, maturation and secretion (PubMed:30771381). Plays a role in NTRK2/TRKB axonal anterograde transport by facilitating the association of NTRK2/TRKB with KLC1 (PubMed:21775604). May be involved in targeting uroplakins to urothelial apical membranes (By similarity).
Cellular Location Membrane; Lipid-anchor. Late endosome
Tissue Location Expressed primarily in testis.
Research Areas
Citations (0)
citation

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Background

May be involved in targeting uroplakins to urothelial apical membranes.

References

Chen D.,et al.Biochem. Mol. Med. 60:27-37(1997).
Chen D.,et al.Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases.
Ramalho J.S.,et al.BMC Genet. 2:2-2(2001).
Puhl H.L. III,et al.Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).

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$ 365.00
$ 140.00
Cat# AM8688b
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