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GDE1 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • WB - GDE1 Antibody AM8683b
    Anti-GDE1 Antibody at 1:2000 dilution + human brain tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 38 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9NZC3
Reactivity Human
Predicted Human
Host Mouse
Clonality monoclonal
Isotype IgG1,κ
Clone/Animal Names 2021CT823.61.7
Calculated MW 37718 Da
Additional Information
Gene ID 51573
Other Names Glycerophosphodiester phosphodiesterase 1, 3.1.4.44, Membrane-interacting protein of RGS16, RGS16-interacting membrane protein, GDE1, MIR16
Target/Specificity This GDE1 antibody is generated from a mouse immunized with a recombinant protein from the human region of human GDE1.
Dilution WB~~1:2000
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGDE1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GDE1 (HGNC:29644)
Function Hydrolyzes the phosphodiester bond of glycerophosphodiesters such as glycerophosphoinositol (GroPIns) and glycerophosphoethanolamine (GroPEth), to yield a glycerol phosphate and an alcohol (By similarity). Hydrolyzes glycerophospho-N-acylethanolamines to N- acylethanolamines in the brain and participates in bioactive N- acylethanolamine biosynthesis such as anandamide (an endocannabinoid), N-palmitoylethanolamine (an anti-inflammatory), and N- oleoylethanolamine (an anorexic). In addition, has a lysophospholipase D activity by hydrolyzing N-acyl-lysoplasmenylethanolamine (N-acyl- lysoPlsEt) to N-acylethanolamine. However lysophospholipase D activity is lower than glycerophosphodiester phosphodiesterase activity (By similarity). Has little or no activity towards glycerophosphocholine (By similarity).
Cellular Location Cell membrane {ECO:0000250|UniProtKB:Q9JL55}; Multi-pass membrane protein. Cytoplasmic vesicle membrane {ECO:0000250|UniProtKB:Q9JL55}; Multi-pass membrane protein. Note=Perinuclear vesicles and cell membrane {ECO:0000250|UniProtKB:Q9JL55}
Tissue Location Widely expressed..
Research Areas
Citations (0)
citation

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Background

Has glycerophosphoinositol phosphodiesterase activity. Has little or no activity towards glycerophosphocholine. GDE1 activity can be modulated by G-protein signaling pathways (By similarity).

References

Zheng B.,et al.Proc. Natl. Acad. Sci. U.S.A. 97:3999-4004(2000).
Duennebier F.F.,et al.Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases.
Loftus B.J.,et al.Genomics 60:295-308(1999).
Bachmann A.S.,et al.Gene 371:144-153(2006).

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$ 365.00
$ 140.00
Cat# AM8683b
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