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MBD2 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • WB - MBD2 Antibody AM8622b
    All lanes : Anti-MBD2 Antibody at 1:2000 dilution Lane 1: K562 whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 43 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - MBD2 Antibody AM8622b
    AM8622b staining MBD2 in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
    detail
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q9UBB5
Reactivity Human
Host Mouse
Clonality monoclonal
Isotype IgG1,k
Clone/Animal Names 1812CT625.9.45
Calculated MW 43255 Da
Additional Information
Gene ID 8932
Other Names Methyl-CpG-binding domain protein 2, Demethylase, DMTase, Methyl-CpG-binding protein MBD2, MBD2
Target/Specificity This MBD2 antibody is generated from a mouse immunized with a recombinant protein between 10-228 amino acids from human MBD2.
Dilution WB~~1:2000
IHC-P~~1:25
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMBD2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MBD2 (HGNC:6917)
Function Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides (PubMed:9774669). Binds hemimethylated DNA as well (PubMed:10947852, PubMed:24307175). Recruits histone deacetylases and DNA methyltransferases to chromatin (PubMed:10471499, PubMed:10947852). Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed:16428440, PubMed:28977666). Acts as a transcriptional repressor and plays a role in gene silencing (PubMed:10471499, PubMed:10947852, PubMed:16415179). Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression (PubMed:16415179). May enhance the activation of some unmethylated cAMP-responsive promoters (PubMed:12665568).
Cellular Location Nucleus. Chromosome Note=Nuclear, in discrete foci (PubMed:12183469). Detected at replication foci in late S phase. Localizes to methylated chromatin (PubMed:16428440). Localizes to sites of DNA damage in a manner partially dependent on ZMYND8 (PubMed:27732854)
Tissue Location Highly expressed in brain, heart, kidney, stomach, testis and placenta.
Research Areas
Citations (0)
citation

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Background

Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression. May enhance the activation of some unmethylated cAMP-responsive promoters.

References

Hendrich B.,et al.Mol. Cell. Biol. 18:6538-6547(1998).
Hendrich B.,et al.Mamm. Genome 10:906-912(1999).
Bhattacharya S.K.,et al.Nature 397:579-583(1999).
Ng H.-H.,et al.Nat. Genet. 23:58-61(1999).
Tatematsu K.,et al.Genes Cells 5:677-688(2000).

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$ 365.00
$ 140.00
Cat# AM8622b
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