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ADK Antibody (N-term)

Purified Mouse Monoclonal Antibody (Mab)

     
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  • WB - ADK Antibody (N-term) AM8619b
    All lanes : Anti-ADK Antibody (N-term) at 1:4000 dilution Lane 1: HepG2 whole cell lysate Lane 2: HL-60 whole cell lysate Lane 3: Human liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - ADK Antibody (N-term) AM8619b
    AM8619b staining ADK in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P55263
Reactivity Human
Host Mouse
Clonality monoclonal
Isotype IgG1,k
Clone/Animal Names 1103CT3.4.3
Calculated MW 40545 Da
Additional Information
Gene ID 132
Other Names Adenosine kinase, AK, 2.7.1.20, Adenosine 5'-phosphotransferase, ADK
Target/Specificity This ADK antibody is generated from a mouse immunized with a recombinant protein between 1-345 amino acids from human ADK.
Dilution WB~~1:4000
IHC-P~~1:25
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsADK Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ADK (HGNC:257)
Function Catalyzes the phosphorylation of the purine nucleoside adenosine at the 5' position in an ATP-dependent manner. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides.
Cellular Location [Isoform 1]: Nucleus
Tissue Location Widely expressed. Highest level in placenta, liver, muscle and kidney.
Research Areas
Citations (0)
citation

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Background

ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides.

References

Spychala J.,et al.Proc. Natl. Acad. Sci. U.S.A. 93:1232-1237(1996).
Singh B.,et al.Eur. J. Biochem. 241:564-571(1996).
McNally T.,et al.Biochem. Biophys. Res. Commun. 231:645-650(1997).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Deloukas P.,et al.Nature 429:375-381(2004).

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$ 365.00
$ 140.00
Cat# AM8619b
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