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PDXK Antibody (N-term)

Purified Mouse Monoclonal Antibody (Mab)

     
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  • WB - PDXK Antibody (N-term) AM8616b
    All lanes : Anti-PDXK Antibody (N-term) at 1:4000 dilution Lane 1: 293 whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: U-251 MG whole cell lysate Lane 4: Human liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 35 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - PDXK Antibody (N-term) AM8616b
    AM8616b staining PDXK in human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession O00764
Reactivity Human
Host Mouse
Clonality monoclonal
Isotype IgG1,k
Clone/Animal Names 1007CT2.1.1
Calculated MW 35102 Da
Additional Information
Gene ID 8566
Other Names Pyridoxal kinase, 2.7.1.35, Pyridoxine kinase, PDXK, C21orf124, C21orf97, PKH, PNK
Target/Specificity This PDXK antibody is generated from a mouse immunized with a recombinant protein between 1-312 amino acids from human PDXK.
Dilution WB~~1:4000
IHC-P~~1:25
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPDXK Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PDXK (HGNC:8819)
Function Catalyzes the phosphorylation of the dietary vitamin B6 vitamers pyridoxal (PL), pyridoxine (PN) and pyridoxamine (PM) to form pyridoxal 5'-phosphate (PLP), pyridoxine 5'-phosphate (PNP) and pyridoxamine 5'-phosphate (PMP), respectively (Probable) (PubMed:10987144, PubMed:17766369, PubMed:19351586, PubMed:31187503, PubMed:9099727). PLP is the active form of vitamin B6, and acts as a cofactor for over 140 different enzymatic reactions.
Cellular Location Cytoplasm, cytosol.
Tissue Location Ubiquitous (PubMed:31187503, PubMed:9099727). Highly expressed in testis (PubMed:9099727)
Research Areas
Citations (0)
citation

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Background

Required for synthesis of pyridoxal-5-phosphate from vitamin B6.

References

Hanna M.C.,et al.J. Biol. Chem. 272:10756-10760(1997).
Fang X.,et al.Asian J. Androl. 6:83-91(2004).
Hattori M.,et al.Nature 405:311-319(2000).
Lee H.-S.,et al.Mol. Cells 10:452-459(2000).
Daub H.,et al.Mol. Cell 31:438-448(2008).

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$ 365.00
$ 140.00
Cat# AM8616b
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