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PFKP Antibody (C-term)

Purified Mouse Monoclonal Antibody (Mab)

     
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  • WB - PFKP Antibody (C-term) AM8580b
    All lanes : Anti-PFKP Antibody (C-term) at 1:4000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: COS-7 whole cell lysate Lane 4: mouse heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 86 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q01813
Reactivity Human, Mouse, Green Monkey
Host Mouse
Clonality monoclonal
Isotype IgG1,k
Clone/Animal Names 1040CT4.6.4.4
Calculated MW 85596 Da
Additional Information
Gene ID 5214
Other Names ATP-dependent 6-phosphofructokinase, platelet type {ECO:0000255|HAMAP-Rule:MF_03184}, ATP-PFK {ECO:0000255|HAMAP-Rule:MF_03184}, PFK-P, 2.7.1.11 {ECO:0000255|HAMAP-Rule:MF_03184}, 6-phosphofructokinase type C, Phosphofructo-1-kinase isozyme C, PFK-C, Phosphohexokinase {ECO:0000255|HAMAP-Rule:MF_03184}, PFKP, PFKF
Target/Specificity This PFKP antibody is generated from a mouse immunized with a recombinant protein between 40-300 amino acids from the C-terminal region of human PFKP.
Dilution WB~~1:4000
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPFKP Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PFKP
Synonyms PFKF
Function Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
Cellular Location Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03184}.
Research Areas
Citations (0)
citation

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Background

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.

References

Eto K.,et al.Biochem. Biophys. Res. Commun. 198:990-998(1994).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Deloukas P.,et al.Nature 429:375-381(2004).
Simpson C.J.,et al.Biochem. Biophys. Res. Commun. 180:197-203(1991).
Rush J.,et al.Nat. Biotechnol. 23:94-101(2005).

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$ 365.00
$ 140.00
Cat# AM8580b
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