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PGK1 Antibody
Purified Mouse Monoclonal Antibody (Mab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, FC, E |
|---|---|
| Primary Accession | P00558 |
| Other Accession | A5A6K4 |
| Reactivity | Human, Mouse |
| Host | Mouse |
| Clonality | monoclonal |
| Isotype | IgG2a,k |
| Clone/Animal Names | 1086CT10.2.1 |
| Calculated MW | 44615 Da |
| Gene ID | 5230 |
|---|---|
| Other Names | Phosphoglycerate kinase 1, 2.7.2.3, Cell migration-inducing gene 10 protein, Primer recognition protein 2, PRP 2, PGK1, PGKA |
| Target/Specificity | This antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 1-417 amino acids from human. |
| Dilution | WB~~1:8000 IHC-P~~1:25 FC~~1:25 |
| Format | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | PGK1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PGK1 |
|---|---|
| Synonyms | PGKA |
| Function | Catalyzes one of the two ATP producing reactions in the glycolytic pathway via the reversible conversion of 1,3- diphosphoglycerate to 3-phosphoglycerate (PubMed:30323285, PubMed:7391028). In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein) (PubMed:2324090). May play a role in sperm motility (PubMed:26677959). |
| Cellular Location | Cytoplasm. |
| Tissue Location | Mainly expressed in spermatogonia. Localized on the principle piece in the sperm (at protein level). Expression significantly decreased in the testis of elderly men |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein).
References
Michelson A.M.,et al.Proc. Natl. Acad. Sci. U.S.A. 80:472-476(1983).
Michelson A.M.,et al.Proc. Natl. Acad. Sci. U.S.A. 82:6965-6969(1985).
Kim J.W.,et al.Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases.
Shichijo S.,et al.Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
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