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RAB27A Antibody
Purified Mouse Monoclonal Antibody (Mab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, FC, E |
|---|---|
| Primary Accession | P51159 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | monoclonal |
| Isotype | IgG1,k |
| Clone/Animal Names | 1590CT813.266.26 |
| Calculated MW | 24868 Da |
| Gene ID | 5873 |
|---|---|
| Other Names | Ras-related protein Rab-27A, Rab-27, GTP-binding protein Ram, RAB27A, RAB27 |
| Target/Specificity | This RAB27A antibody is generated from a mouse immunized with a recombinant protein of human RAB27A. |
| Dilution | WB~~1:2000 FC~~1:25 |
| Format | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | RAB27A Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | RAB27A |
|---|---|
| Synonyms | RAB27 |
| Function | Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate homeostasis of late endocytic pathway, including endosomal positioning, maturation and secretion (PubMed:30771381). Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse. |
| Cellular Location | Membrane; Lipid-anchor. Melanosome. Late endosome. Lysosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:12643545, PubMed:17081065). Localizes to endosomal exocytic vesicles (PubMed:17237785). |
| Tissue Location | Found in all the examined tissues except in brain. Low expression was found in thymus, kidney, muscle and placenta Detected in melanocytes, and in most tumor cell lines examined Expressed in cytotoxic T-lymphocytes (CTL) and mast cells |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse.
References
Seabra M.C.,et al.J. Biol. Chem. 270:24420-24427(1995).
Seabra M.C.,et al.Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases.
Chen D.,et al.Biochem. Mol. Med. 60:27-37(1997).
Tolmachova T.,et al.Gene 239:109-116(1999).
Hu R.-M.,et al.Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000).
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