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> home > Products > Primary Antibodies > Antibody Collections > Loading Control > GAPDH Antibody, HRP Conjugate
GAPDH Antibody, HRP Conjugate
Purified Mouse Monoclonal Antibody (Mab)
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Application 
| WB, E |
|---|---|
| Primary Accession | P04406 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | monoclonal |
| Isotype | IgG1 |
| Clone/Animal Names | 1653CT401.3.33 |
| Calculated MW | 36053 Da |
| Gene ID | 2597 |
|---|---|
| Other Names | GAPD |
| Target/Specificity | This GAPDH antibody is generated from a mouse immunized with a recombinant protein of human GAPDH. |
| Dilution | WB~~1:1000 |
| Format | Purified monoclonal antibody conjugated with HRP supplied in PBS with 0.05% (W/V) Proclin 300. This antibody is purified through a protein G column, followed by size-exclusion - Membrane filtration purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | GAPDH Antibody, HRP Conjugate is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | GAPDH {ECO:0000303|PubMed:2987855, ECO:0000312|HGNC:HGNC:4141} |
|---|---|
| Function | Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively (PubMed:11724794, PubMed:3170585). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D- glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate (PubMed:11724794, PubMed:3170585). Modulates the organization and assembly of the cytoskeleton (By similarity). Facilitates the CHP1- dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes (PubMed:23071094). Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (PubMed:23071094). Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (PubMed:23332158, PubMed:27387501). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis (By similarity). Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). |
| Cellular Location | Cytoplasm, cytosol. Nucleus {ECO:0000250|UniProtKB:P04797}. Cytoplasm, perinuclear region. Membrane Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:P04797} Note=Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions (PubMed:12829261) {ECO:0000250|UniProtKB:P04797, ECO:0000269|PubMed:12829261} |
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$ 365.00
$ 140.00
Cat# AM8501b
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