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DENR Antibody
Purified Mouse Monoclonal Antibody (Mab)
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- CITATIONS
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- BACKGROUND
Application 
| WB, IHC-P, FC, IF, E |
|---|---|
| Primary Accession | O43583 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | monoclonal |
| Isotype | IgG2b,κ |
| Clone/Animal Names | 1542CT106.51.79 |
| Calculated MW | 22092 Da |
| Gene ID | 8562 |
|---|---|
| Other Names | Density-regulated protein, DRP, Protein DRP1, Smooth muscle cell-associated protein 3, SMAP-3, DENR, DRP1 |
| Target/Specificity | This DENR antibody is generated from a mouse immunized with a recombinant protein of human DENR. |
| Dilution | IF~~1:25 WB~~1:1000-1:2000 IHC-P~~1:25 FC~~1:25 |
| Format | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | DENR Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | DENR |
|---|---|
| Synonyms | DRP1 |
| Function | Translation regulator forming a complex with MCTS1 to promote translation reinitiation. Translation reinitiation is the process where the small ribosomal subunit remains attached to the mRNA following termination of translation of a regulatory upstream ORF (uORF), and resume scanning on the same mRNA molecule to initiate translation of a downstream ORF, usually the main ORF (mORF). The MCTS1/DENR complex is pivotal to two linked mechanisms essential for translation reinitiation. Firstly, the dissociation of deacylated tRNAs from post- termination 40S ribosomal complexes during ribosome recycling. Secondly, the recruitment in an EIF2-independent manner of aminoacylated initiator tRNA to P site of 40S ribosomes for a new round of translation. This regulatory mechanism governs the translation of more than 150 genes which translation reinitiation is MCTS1/DENR complex-dependent. |
| Cellular Location | Cytoplasm. |
| Tissue Location | Highly expressed in heart and skeletal muscle and moderately expressed in the brain, placenta, liver and pancreas. Weakly expressed in the lung and kidney. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
May be involved in the translation of target mRNAs by scanning and recognition of the initiation codon. Involved in translation initiation; promotes recruitmnet of aminoacetyled initiator tRNA to P site of 40S ribosomes. Can promote release of deacylated tRNA and mRNA from recycled 40S subunits following ABCE1-mediated dissociation of post-termination ribosomal complexes into subunits. Plays a role in the modulation of the translational profile of a subset of cancer-related mRNAs when recruited to the translational initiation complex by the oncogene MCTS1.
References
Deyo J.E.,et al.DNA Cell Biol. 17:437-447(1998).
Nishimoto S.,et al.Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases.
Scherer S.E.,et al.Nature 440:346-351(2006).
Oh J.J.,et al.Nucleic Acids Res. 27:4008-4017(1999).
Reinert L.S.,et al.Cancer Res. 66:8994-9001(2006).
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