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CDK4 Antibody
Purified Mouse Monoclonal Antibody (Mab)
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Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | P11802 |
| Reactivity | Human, Mouse |
| Host | Mouse |
| Clonality | monoclonal |
| Isotype | IgG2a,κ |
| Clone/Animal Names | 1529CT850.162.73 |
| Calculated MW | 33730 Da |
| Gene ID | 1019 |
|---|---|
| Other Names | Cyclin-dependent kinase 4, Cell division protein kinase 4, PSK-J3, CDK4 |
| Target/Specificity | This CDK4 antibody is generated from a mouse immunized with a recombinant protein. |
| Dilution | WB~~1:2000 IHC~~1:300 IHC-P~~1:25 |
| Format | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | CDK4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CDK4 |
|---|---|
| Function | Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. |
| Cellular Location | Cytoplasm. Nucleus. Nucleus membrane. Note=Cytoplasmic when non-complexed Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G(1) phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G(1) to S phase. Also present in nucleoli and heterochromatin lumps. Colocalizes with RB1 after release into the nucleus. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
References
Hanks S.K.,et al.Submitted (FEB-1987) to the EMBL/GenBank/DDBJ databases.
Elkahloun A.G.,et al.Genomics 42:295-301(1997).
Wolfel T.,et al.Science 269:1281-1284(1995).
Zuo L.,et al.Nat. Genet. 12:97-99(1996).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
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