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VWF Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • WB - VWF Antibody AM8430b
    Anti-VWF at dilution + VWF whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 309 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - VWF Antibody AM8430b
    Immunohistochemical analysis of paraffin-embedded H. kidney section using VWF(Cat#AM8430b). AM8430b was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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  • FC - VWF Antibody AM8430b
    Flow cytometric analysis of K562 cells using VWF(green, Cat#AM8430b) compared to an isotype control of mouse IgG1(blue). AP20600c was diluted at 1:100 dilution. An Alexa Fluor® 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.
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  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, IHC-P, E
Primary Accession P04275
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone/Animal Names 907CT12.1.9
Calculated MW 309265 Da
Additional Information
Gene ID 7450
Other Names von Willebrand factor, vWF, von Willebrand antigen 2, von Willebrand antigen II, VWF, F8VWF
Target/Specificity This antibody is generated from a mouse immunized with .VWF recombinant protein.
Dilution WB~~1:2000
IHC-P~~1:25
FC~~1:100
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsVWF Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name VWF
Synonyms F8VWF
Function Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet- surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
Cellular Location Secreted. Secreted, extracellular space, extracellular matrix. Note=Localized to storage granules
Tissue Location Plasma.
Research Areas
Citations (0)
citation

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Background

Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.

References

Bonthron D.,et al.Nucleic Acids Res. 14:7125-7128(1986).
Mancuso D.J.,et al.J. Biol. Chem. 264:19514-19527(1989).
Scherer S.E.,et al.Nature 440:346-351(2006).
Verweij C.L.,et al.EMBO J. 5:1839-1847(1986).
Verweij C.L.,et al.EMBO J. 5:3074-3074(1986).

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$ 365.00
$ 140.00
Cat# AM8430b
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