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CAPN1 Antibody
Purified Mouse Monoclonal Antibody (Mab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, FC, WB, E |
|---|---|
| Primary Accession | P07384 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1,k |
| Clone/Animal Names | 1376CT809.24.85 |
| Calculated MW | 81890 Da |
| Antigen Region | 1-420 aa |
| Gene ID | 823 |
|---|---|
| Other Names | Calpain-1 catalytic subunit, Calcium-activated neutral proteinase 1, CANP 1, Calpain mu-type, Calpain-1 large subunit, Cell proliferation-inducing gene 30 protein, Micromolar-calpain, muCANP, CAPN1, CANPL1 |
| Target/Specificity | This CAPN1 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between amino acids from the human region of human CAPN1. |
| Dilution | WB~~1:1000 IHC-P~~1:25 FC~~1:25 |
| Format | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | CAPN1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CAPN1 (HGNC:1476) |
|---|---|
| Synonyms | CANPL1 |
| Function | Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction (PubMed:19617626, PubMed:21531719, PubMed:2400579). Proteolytically cleaves CTBP1 at 'Asn-375', 'Gly-387' and 'His-409' (PubMed:23707407). Cleaves and activates caspase-7 (CASP7) (PubMed:19617626). |
| Cellular Location | Cytoplasm. Cell membrane. Note=Translocates to the plasma membrane upon Ca(2+) binding. In granular keratinocytes and in lower corneocytes, colocalizes with FLG and FLG2 (PubMed:21531719) |
| Tissue Location | Ubiquitous. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
References
Aoki K.,et al.FEBS Lett. 205:313-317(1986).
Sorimachi H.,et al.Biol. Chem. Hoppe-Seyler 371:171-176(1990).
Kim J.W.,et al.Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.
Melloni E.,et al.Biochem. Biophys. Res. Commun. 229:193-197(1996).
Michetti M.,et al.FEBS Lett. 392:11-15(1996).
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