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MAFK Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • WB - MAFK Antibody AM8420b
    Western blot analysis of lysate from Jurkat cell line, using MAFK Antibody(Cat. #AM8420b). AM8420b was diluted at 1:1000. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 35μg.
    detail
  • FC - MAFK Antibody AM8420b
    Flow cytometric analysis of Hela cells using MAFK Antibody(green, Cat#AM8420b) compared to an isotype control of mouse IgG1(blue). AM8420b was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
FC, WB, E
Primary Accession O60675
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1,κ
Clone/Animal Names 1328CT786.105.125
Calculated MW 17523 Da
Additional Information
Gene ID 7975
Other Names Transcription factor MafK, Erythroid transcription factor NF-E2 p18 subunit, MAFK
Target/Specificity This MAFK antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between amino acids from the human region of human MAFK.
Dilution WB~~1:1000
FC~~1:25
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMAFK Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MAFK
Function Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves (PubMed:9150357). However, they act as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins, such as NFE2, NFE2L1/NRF1, NFE2L2/NRF2 and NFE2L3/NRF3, and recruiting them to specific DNA-binding sites (PubMed:8932385, PubMed:9150357). Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor (PubMed:9150357).
Cellular Location Nucleus.
Research Areas
Citations (0)
citation

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Background

Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor.

References

Toki T.,et al.Oncogene 14:1901-1910(1997).
Hillier L.W.,et al.Nature 424:157-164(2003).
Daub H.,et al.Mol. Cell 31:438-448(2008).
Dephoure N.,et al.Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
Mayya V.,et al.Sci. Signal. 2:RA46-RA46(2009).

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$ 365.00
$ 140.00
Cat# AM8420b
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