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PAH Antibody (Center)

Purified Mouse Monoclonal Antibody (Mab)

     
  • WB - PAH Antibody (Center) AM8419b
    All lanes: Anti-PAH Antibody (Center) at 1:1000 dilution Lane 1: C6 whole cell lysate Lane 2: Rat brain lysate Lysates/proteins at 20 µg per lane. Secondary: Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated (ASP1614) at 1/8000 dilution. Observed band size: 52 KDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - PAH Antibody (Center) AM8419b
    All lanes: Anti-PAH Antibody (Center) at 1:1000 dilution Lane 1: C6 whole cell lysate Lane 2: Rat brain lysate Lysates/proteins at 20 µg per lane. Secondary: Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated (ASP1613) at 1/8000 dilution. Observed band size: 52 KDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - PAH Antibody (Center) AM8419b
    All lanes: Anti-PAH Antibody (Center) at 1:1000 dilution Lane 1: C6 whole cell lysate Lane 2: Rat brain lysate Lysates/proteins at 20 µg per lane. Secondary: Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated (ASP1613) at 1/8000 dilution. Observed band size: 52 KDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - PAH Antibody (Center) AM8419b
    Western blot analysis of lysates from HepG2 cell line and mouse liver rat liver tissue lysates (from left to right) using PAH Antibody (Center)(Cat. #AM8419b). AM8419b was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.
    detail
  • IHC-P - PAH Antibody (Center) AM8419b
    Immunohistochemical analysis of paraffin-embedded H. liver section using PAH Antibody (Center)(Cat#AM8419b). AM8419b was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
    detail
  • IHC-P - PAH Antibody (Center) AM8419b
    Immunohistochemical analysis of paraffin-embedded M. kidney section using PAH Antibody (Center)(Cat#AM8419b). AM8419b was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
    detail
  • IHC-P - PAH Antibody (Center) AM8419b
    AM8419b staining PAH in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P00439
Other Accession P04176, P16331, Q2KIH7
Reactivity Human, Mouse, Rat
Predicted Bovine
Host Mouse
Clonality Monoclonal
Isotype IgG1,κ
Clone/Animal Names 717CT21.1.1
Calculated MW 51862 Da
Additional Information
Gene ID 5053
Other Names Phenylalanine-4-hydroxylase, PAH, Phe-4-monooxygenase, PAH
Target/Specificity This PAH antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 127-161 amino acids from the Central region of human PAH.
Dilution WB~~1:1000
IHC-P~~1:25
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPAH Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PAH
Function Catalyzes the hydroxylation of L-phenylalanine to L-tyrosine.
Research Areas
Citations ( 0 )

References

Kwok S.C.M.,et al.Biochemistry 24:556-561(1985).
Scriver C.R.,et al.Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases.
Cotton R.G.,et al.Biochem. J. 255:193-196(1988).
Miranda F.F.,et al.J. Biol. Chem. 277:40937-40943(2002).
Siltberg-Liberles J.,et al.Gene 427:86-92(2008).

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$ 365.00
$ 140.00
Cat# AM8419b
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