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CD73(NT5E) Antibody (C-term)
Mouse Monoclonal Antibody (Mab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | P21589 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone/Animal Names | 974CT7.5.3 |
| Calculated MW | 63368 Da |
| Gene ID | 4907 |
|---|---|
| Other Names | 5'-nucleotidase, 5'-NT, Ecto-5'-nucleotidase, CD73, NT5E, NT5, NTE |
| Target/Specificity | Purified His-tagged CD73(NT5E) protein was used to produced this monoclonal antibody. |
| Dilution | WB~~1:2000 IHC-P~~1:25 |
| Format | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | CD73(NT5E) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | NT5E |
|---|---|
| Synonyms | NT5, NTE |
| Function | Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate (PubMed:21933152, PubMed:22997138, PubMed:23142347, PubMed:24887587, PubMed:34403084). Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms (PubMed:34403084). Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN (PubMed:21933152, PubMed:22997138, PubMed:23142347, PubMed:24887587, PubMed:34403084). |
| Cellular Location | Cell membrane; Lipid-anchor, GPI-anchor |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
References
Misumi Y., et al. Eur. J. Biochem. 191:563-569(1990).
Otsuki T., et al. DNA Res. 12:117-126(2005).
Mungall A.J., et al. Nature 425:805-811(2003).
Hansen K.R., et al. Gene 167:307-312(1995).
Zanoni L., et al. Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases.
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