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UCH37 (UCHL5) Antibody

Mouse Monoclonal Antibody (Mab)

     
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  • WB - UCH37 (UCHL5) Antibody AM2200b
    All lanes : Anti-UCH37 (UCHL5) at 1:2000-1:4000 dilution Lane 1: 293 whole cell lysate Lane 2: NCI-H460 whole cell lysate Lane 3: HeLa whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9Y5K5
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone/Animal Names 854CT5.5.6
Calculated MW 37607 Da
Additional Information
Gene ID 51377
Other Names Ubiquitin carboxyl-terminal hydrolase isozyme L5, UCH-L5, Ubiquitin C-terminal hydrolase UCH37, Ubiquitin thioesterase L5, UCHL5, UCH37
Target/Specificity Purified His-tagged UCH37 protein was used to produced this monoclonal antibody.
Dilution WB~~1:2000-1:4000
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUCH37 (UCHL5) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name UCHL5
Synonyms UCH37
Function Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Putative regulatory component of the INO80 complex; however is inactive in the INO80 complex and is activated by a transient interaction of the INO80 complex with the proteasome via ADRM1.
Cellular Location Cytoplasm. Nucleus. Note=Associates with the proteasome 19S subunit in the cytoplasm. Associates with the INO80 complex in the nucleus
Research Areas
Citations (0)
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Background

Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Putative regulatory component of the INO80 complex; however is inactive in the INO80 complex and is activated by a transient interaction of the INO80 complex with the proteasome via ADRM1.

References

Yao T., et al. Nat. Cell Biol. 8:994-1002(2006).
Lai C.-H., et al. Genome Res. 10:703-713(2000).
Hu R.-M., et al. Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000).
Kalnine N., et al. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Gregory S.G., et al. Nature 441:315-321(2006).

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$ 365.00
$ 140.00
Cat# AM2200b
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