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CDH1 Antibody
Mouse Monoclonal Antibody (Mab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | P12830 |
| Reactivity | Human, Mouse |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1,k |
| Clone/Animal Names | 813CT11.1.3 |
| Calculated MW | 97456 Da |
| Gene ID | 999 |
|---|---|
| Other Names | Cadherin-1, CAM 120/80, Epithelial cadherin, E-cadherin, Uvomorulin, CD324, E-Cad/CTF1, E-Cad/CTF2, E-Cad/CTF3, CDH1, CDHE, UVO |
| Target/Specificity | Purified His-tagged CDH1 protein was used to produced this monoclonal antibody. |
| Dilution | WB~~1:4000 IHC-P~~1:25 |
| Format | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | CDH1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CDH1 (HGNC:1748) |
|---|---|
| Function | Cadherins are calcium-dependent cell adhesion proteins (PubMed:11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed:11976333). Promotes organization of radial actin fiber structure and cellular response to contractile forces, via its interaction with AMOTL2 which facilitates anchoring of radial actin fibers to CDH1 junction complexes at the cell membrane (By similarity). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. |
| Cellular Location | Cell junction, adherens junction. Cell membrane; Single-pass type I membrane protein Endosome. Golgi apparatus, trans-Golgi network. Cytoplasm {ECO:0000250|UniProtKB:P09803}. Cell junction, desmosome. Note=Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma- catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane. Recruited to desmosomes at the initial assembly phase and also accumulates progressively at mature desmosome cell-cell junctions (PubMed:25208567) |
| Tissue Location | Expressed in granuloma macrophages (at protein level) (PubMed:27760340). Expressed in the liver (PubMed:3263290) |
Provided below are standard protocols that you may find useful for product applications.
Background
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.
References
Bussemakers M.J.G., et al. Mol. Biol. Rep. 17:123-128(1993).
Oda T., et al. Proc. Natl. Acad. Sci. U.S.A. 91:1858-1862(1994).
Rimm D.L., et al. Biochem. Biophys. Res. Commun. 200:1754-1761(1994).
Ito K., et al. Oncogene 18:7080-7090(1999).
Bussemakers M.J.G., et al. Biochem. Biophys. Res. Commun. 203:1284-1290(1994).
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