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CD138 Antibody

Mouse Monoclonal Antibody (Mab)

     
  • IF - CD138 Antibody AM2157b
    Confocal immunofluorescent analysis of CD138 antibody (Cat#AM2157a) with RPMI8266 cell (above) compared with Jurkat as negative cell line (below).followed by DyLight 488-conjugated goat anti-mouse lgG (H+L) Secondary Antibody (green).DAPI was used to stain the cell nucleus (blue).
    detail
  • WB - CD138 Antibody AM2157b
    CD138 Antibody (Cat. #AM2157a) western blot analysis in T47D cell line lysates (35μg/lane).This demonstrates the CD138 antibody detected the CD138 protein (arrow).
    detail
  • FC - CD138 Antibody AM2157b
    CD138 Antibody flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).Alexa Fluor® 488-conjugated donkey anti-mouse lgG secondary antibodies were used for the analysis
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IF, FC, E
Primary Accession P18827
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone/Animal Names 480CT5.4.3
Calculated MW 32462 Da
Additional Information
Gene ID 6382
Other Names Syndecan-1, SYND1, CD138, SDC1, SDC
Target/Specificity This CD138 Monoclonal antibody is generated from mouses immunized with a KLH conjugated synthetic peptide selected from human CD138.
Dilution IF~~1:10~50
WB~~1:500~1000
FC~~1:10~50
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD138 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SDC1 (HGNC:10658)
Synonyms SDC
Function Cell surface proteoglycan that contains both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix (By similarity). Regulates exosome biogenesis in concert with SDCBP and PDCD6IP (PubMed:22660413). Able to induce its own expression in dental mesenchymal cells and also in the neighboring dental epithelial cells via an MSX1-mediated pathway (By similarity).
Cellular Location Membrane; Single-pass type I membrane protein. Secreted Secreted, extracellular exosome Note=Shedding of the ectodomain produces a soluble form
Tissue Location Detected in placenta (at protein level) (PubMed:32337544). Detected in fibroblasts (at protein level) (PubMed:36213313).
Research Areas
Citations (0)
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Background

The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same protein.

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$ 365.00
$ 140.00
Cat# AM2157b
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