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MyoD1 Antibody (N-term) (Ascites)

Mouse Monoclonal Antibody (Mab)

     
  • WB - MyoD1 Antibody (N-term) (Ascites) AM2147a
    MyoD1 Antibody (N-term)(Ascites)(Cat. #AM2147a) western blot analysis in A549 cell line lysates (35μg/lane).This demonstrates the MyoD1 antibody detected the MyoD1 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P15172
Other Accession P13904, Q02346, P49811, P10085, P16075, Q7YS82, NP_002469, P29331
Reactivity Human
Predicted Bovine, Chicken, Mouse, Pig, Rat, Sheep, Xenopus
Host Mouse
Clonality Monoclonal
Isotype IgM
Clone/Animal Names 598CT21.2.4
Calculated MW 34501 Da
Antigen Region 105-134 aa
Additional Information
Gene ID 4654
Other Names Myoblast determination protein 1, Class C basic helix-loop-helix protein 1, bHLHc1, Myogenic factor 3, Myf-3, MYOD1, BHLHC1, MYF3, MYOD
Target/Specificity This MyoD1 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 105-134 amino acids from the N-terminal region of human MyoD1.
Dilution WB~~1:100~2000
Format Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMyoD1 Antibody (N-term) (Ascites) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MYOD1
Synonyms BHLHC1, MYF3, MYOD
Function Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins (By similarity).
Cellular Location Nucleus.
Research Areas
Citations (0)
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Background

This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis.

References

Xynos, A., et al. Stem Cells 28(5):965-973(2010)
Stuelsatz, P., et al. J. Biol. Chem. 285(17):12670-12683(2010)
Hiraoka, S., et al. Hum. Pathol. 41(1):38-47(2010)
Yerges, L.M., et al. J. Bone Miner. Res. 24(12):2039-2049(2009)
Yang, Z., et al. Genes Dev. 23(6):694-707(2009)

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$ 182.50
Cat# AM2147a
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