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IL2 Antibody (Center) (Ascites)

Mouse Monoclonal Antibody (Mab)

     
  • WB - IL2 Antibody (Center) (Ascites) AM2117a
    IL2 Antibody (Center)(Ascites)(Cat. #AM2117a) western blot analysis in K562 cell line lysates (35μg/lane).This demonstrates the IL2 antibody detected the IL2 protein (arrow).
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P60568
Other Accession NP_000577.2
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone/Animal Names 627CT14.8.1
Calculated MW 17628 Da
Antigen Region 50-77 aa
Additional Information
Gene ID 3558
Other Names Interleukin-2, IL-2, T-cell growth factor, TCGF, Aldesleukin, IL2
Target/Specificity This IL2 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 50-77 amino acids from the Central region of human IL2.
Dilution WB~~1:100~1600
Format Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsIL2 Antibody (Center) (Ascites) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IL2
Function Cytokine produced by activated CD4-positive helper T-cells and to a lesser extend activated CD8-positive T-cells and natural killer (NK) cells that plays pivotal roles in the immune response and tolerance (PubMed:6438535). Binds to a receptor complex composed of either the high-affinity trimeric IL-2R (IL2RA/CD25, IL2RB/CD122 and IL2RG/CD132) or the low-affinity dimeric IL-2R (IL2RB and IL2RG) (PubMed:16293754, PubMed:16477002). Interaction with the receptor leads to oligomerization and conformation changes in the IL-2R subunits resulting in downstream signaling starting with phosphorylation of JAK1 and JAK3 (PubMed:7973659). In turn, JAK1 and JAK3 phosphorylate the receptor to form a docking site leading to the phosphorylation of several substrates including STAT5 (PubMed:8580378). This process leads to activation of several pathways including STAT, phosphoinositide-3- kinase/PI3K and mitogen-activated protein kinase/MAPK pathways (PubMed:25142963). Functions as a T-cell growth factor and can increase NK-cell cytolytic activity as well (PubMed:6608729). Promotes strong proliferation of activated B-cells and subsequently immunoglobulin production (PubMed:6438535). Plays a pivotal role in regulating the adaptive immune system by controlling the survival and proliferation of regulatory T-cells, which are required for the maintenance of immune tolerance. Moreover, participates in the differentiation and homeostasis of effector T-cell subsets, including Th1, Th2, Th17 as well as memory CD8-positive T-cells.
Cellular Location Secreted.
Research Areas
Citations (0)
citation

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Background

The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.

References

Zhu, P., et al. J. Immunol. 185(9):5140-5149(2010)
Muller, T., et al. Scand. J. Immunol. 72(4):365-371(2010)
Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) :
Horowitz, A., et al. J. Immunol. 185(5):2808-2818(2010)
Wu, Z., et al. J Mol Cell Biol 2(4):217-222(2010)

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$ 182.50
Cat# AM2117a
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