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EPCAM Antibody
Mouse Monoclonal Antibody (Mab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P-Leica, E |
|---|---|
| Primary Accession | P16422 |
| Other Accession | NP_002345.2 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone/Animal Names | 463CT25.1.1 |
| Calculated MW | 34932 Da |
| Antigen Region | 59-86 aa |
| Gene ID | 4072 |
|---|---|
| Other Names | Epithelial cell adhesion molecule, Ep-CAM, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein, EGP, Epithelial glycoprotein 314, EGP314, hEGP314, KS 1/4 antigen, KSA, Major gastrointestinal tumor-associated protein GA733-2, Tumor-associated calcium signal transducer 1, CD326, EPCAM, GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1 |
| Target/Specificity | This EPCAM antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 59-86 amino acids from human EPCAM. |
| Dilution | WB~~1:8000 IHC-P-Leica~~1:1000 |
| Format | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | EPCAM Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | EPCAM |
|---|---|
| Synonyms | GA733-2, M1S2, M4S1, MIC18, TACSTD1, TRO |
| Function | May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E. |
| Cellular Location | Lateral cell membrane; Single-pass type I membrane protein. Cell junction, tight junction. Note=Colocalizes with CLDN7 at the lateral cell membrane and tight junction |
| Tissue Location | Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC) Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma. |
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy.
References
Kimura, O., et al. Cancer Sci. 101(10):2145-2155(2010)
Jiang, L., et al. Breast Cancer Res. Treat. (2010) In press :
Lugli, A., et al. Br. J. Cancer 103(3):382-390(2010)
Johnatty, S.E., et al. PLoS Genet. 6 (7), E1001016 (2010) :
Ren, G., et al. Zhonghua Zhong Liu Za Zhi 31(11):841-844(2009)
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