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SPRED1 Antibody
Mouse Monoclonal Antibody (Mab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q7Z699 |
| Other Accession | NP_689807.1 |
| Reactivity | Human, Mouse |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone/Animal Names | 462CT2.5.1 |
| Calculated MW | 50477 Da |
| Gene ID | 161742 |
|---|---|
| Other Names | Sprouty-related, EVH1 domain-containing protein 1, Spred-1, hSpred1, SPRED1 |
| Target/Specificity | Purified His-tagged SPRED1 protein(Fragment) was used to produced this monoclonal antibody. |
| Dilution | WB~~1:500~1000 |
| Format | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | SPRED1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SPRED1 |
|---|---|
| Function | Tyrosine kinase substrate that inhibits growth-factor- mediated activation of MAP kinase (By similarity). Negatively regulates hematopoiesis of bone marrow (By similarity). Inhibits fibroblast growth factor (FGF)-induced retinal lens fiber differentiation, probably by inhibiting FGF-mediated phosphorylation of ERK1/2 (By similarity). Attenuates actin stress fiber formation via inhibition of TESK1-mediated phosphorylation of cofilin (PubMed:18216281). Inhibits TGFB-induced epithelial-to-mesenchymal transition in lens epithelial cells (By similarity). |
| Cellular Location | Cell membrane; Peripheral membrane protein. Membrane, caveola; Peripheral membrane protein. Nucleus Note=Localized in cholesterol-rich membrane raft/caveola fractions |
| Tissue Location | Weakly expressed in embryonic cell line HEK293. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
The protein encoded by this gene is a member of the Sprouty family of proteins and is phosphorylated by tyrosine kinase in response to several growth factors. The encoded protein can act as a homodimer or as a heterodimer with SPRED2 to regulate activation of the MAP kinase cascade. Defects in this gene are a cause of neurofibromatosis type 1-like syndrome (NFLS). [provided by RefSeq].
References
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Batz, C., et al. Blood 115(12):2557-2558(2010)
Messiaen, L., et al. JAMA 302(19):2111-2118(2009)
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Spurlock, G., et al. J. Med. Genet. 46(7):431-437(2009)
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