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T Antibody (Ascites)

Mouse Monoclonal Antibody (Mab)

     
  • WB - T Antibody (Ascites) AM1993a
    T Antibody (Cat. #AM1993a) western blot analysis in NCI-H292 cell line lysates (35μg/lane).This demonstrates the T antibody detected the T protein (arrow).
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  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession O15178
Other Accession Q07998, NP_003172.1
Reactivity Human
Predicted Zebrafish
Host Mouse
Clonality Monoclonal
Isotype IgM
Clone/Animal Names 394CT14.1.4
Calculated MW 47443 Da
Antigen Region 201-228 aa
Additional Information
Gene ID 6862
Other Names Brachyury protein, Protein T, T
Target/Specificity This T antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 201-228 amino acids from human T.
Dilution WB~~1:1000~8000
Format Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsT Antibody (Ascites) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TBXT (HGNC:11515)
Synonyms T
Function Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to a palindromic T site 5'-TTCACACCTAGGTGTGAA-3' DNA sequence and activates gene transcription when bound to such a site.
Cellular Location Nucleus
Tissue Location Detected in testis, but not in other, normal tissues. Detected in lung tumors (at protein level)
Research Areas
Citations (0)
citation

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Background

The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells.

References

Jambhekar, N.A., et al. Arch. Pathol. Lab. Med. 134(8):1181-1187(2010)
Docherty, S.J., et al. BMC Genet. 11, 61 (2010) :
Yang, X.R., et al. Nat. Genet. 41(11):1176-1178(2009)
Oakley, G.J., et al. Mod. Pathol. 21(12):1461-1469(2008)
Bondue, A., et al. Cell Stem Cell 3(1):69-84(2008)

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$ 182.50
Cat# AM1993a
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