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> home > Products > Primary Antibodies > Antibody Collections > mRNA processing > THOC1 Antibody (N-term)
THOC1 Antibody (N-term)
Mouse Monoclonal Antibody (Mab)
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- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q96FV9 |
| Other Accession | Q8R3N6, NP_005122.2 |
| Reactivity | Human |
| Predicted | Mouse |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG3,k |
| Clone/Animal Names | 301CT40.5.1 |
| Calculated MW | 75666 Da |
| Antigen Region | 257-285 aa |
| Gene ID | 9984 |
|---|---|
| Other Names | THO complex subunit 1, Tho1, Nuclear matrix protein p84, p84N5, hTREX84, THOC1, HPR1 |
| Target/Specificity | This THOC1 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 257-285 amino acids from the N-terminal region of human THOC1. |
| Dilution | WB~~1:500~1000 |
| Format | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | THOC1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | THOC1 |
|---|---|
| Synonyms | HPR1 |
| Function | Component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA (PubMed:15833825, PubMed:15998806, PubMed:17190602). Required for efficient export of polyadenylated RNA (PubMed:23222130). The THOC1-THOC2-THOC3 core complex alone is sufficient to bind export factor NXF1-NXT1 and promote ATPase activity of DDX39B/UAP56 (PubMed:33191911). TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap- dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NXF1 pathway (PubMed:15833825, PubMed:15998806, PubMed:17190602). Regulates transcriptional elongation of a subset of genes (PubMed:22144908). Involved in genome stability by preventing co-transcriptional R-loop formation (By similarity). May play a role in hair cell formation, hence may be involved in hearing (By similarity). |
| Cellular Location | [Isoform 1]: Nucleus speckle. Nucleus, nucleoplasm. Nucleus matrix. Cytoplasm. Note=Can shuttle between the nucleus and cytoplasm. Nuclear localization is required for induction of apoptotic cell death. Translocates to the cytoplasm during the early phase of apoptosis execution |
| Tissue Location | Ubiquitous. Expressed in various cancer cell lines. Expressed at very low levels in normal breast epithelial cells and highly expressed in breast tumors. Expression is strongly associated with an aggressive phenotype of breast tumors and expression correlates with tumor size and the metastatic state of the tumor progression |
Research Areas
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
Background
HPR1 is part of the TREX (transcription/export) complex, which includes TEX1 (MIM 606929), THO2 (MIM 300395), ALY (MIM 604171), and UAP56 (MIM 142560).
References
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Liu, Y., et al. Mol. Psychiatry (2010) In press :
Boyne, J.R., et al. PLoS Pathog. 4 (10), E1000194 (2008) :
Ferreira, M.A., et al. Nat. Genet. 40(9):1056-1058(2008)
Yang, J., et al. Ann. Clin. Lab. Sci. 38(2):105-112(2008)
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$ 192.50
$ 75.00
Cat# AM1950b
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