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MAP4K3 Antibody
Mouse Monoclonal Antibody (Mab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | Q8IVH8 |
| Other Accession | NP_003609.2 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgM,k |
| Clone/Animal Names | 219CT8.3.1 |
| Calculated MW | 101316 Da |
| Gene ID | 8491 |
|---|---|
| Other Names | Mitogen-activated protein kinase kinase kinase kinase 3, Germinal center kinase-related protein kinase, GLK, MAPK/ERK kinase kinase kinase 3, MEK kinase kinase 3, MEKKK 3, MAP4K3, RAB8IPL1 |
| Target/Specificity | This MAP4K3 monoclonal antibody is generated from mouse immunized with MAP4K3 recombinant protein. |
| Dilution | WB~~1:1000 IHC-P~~1:100 |
| Format | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Euglobin precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | MAP4K3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | MAP4K3 (HGNC:6865) |
|---|---|
| Synonyms | RAB8IPL1 |
| Function | Serine/threonine kinase that plays a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway (PubMed:9275185). Activator of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. MAP4Ks act in parallel to and are partially redundant with STK3/MST2 and STK4/MST2 in the phosphorylation and activation of LATS1/2, and establish MAP4Ks as components of the expanded Hippo pathway (PubMed:26437443). |
| Tissue Location | Ubiquitously expressed in all tissues examined, with high levels in heart, brain, placenta, skeletal muscle, kidney and pancreas and lower levels in lung and liver |
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes a member of the Ste20 family of serine/threonine protein kinases. The protein belongs to the subfamily that consists of members, such as germinal center kinase (GCK), that are characterized by an N-terminal catalytic domain and C-terminal regulatory domain. The kinase activity of the encoded protein can be stimulated by UV radiation and tumor necrosis factor-alpha. The protein specifically activates the c-Jun N-terminal kinase (JNK) signaling pathway. Evidence suggests that it functions upstream of mitogen-activated protein kinase kinase kinase 1 (MEKK1). This gene previously was referred to as RAB8-interacting protein-like 1 (RAB8IPL1), but it has been renamed mitogen-activated protein kinase kinase kinase kinase 3 (MAP4K3).
References
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Ichikawa, S., et al. J. Bone Miner. Res. 25(8):1821-1829(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Yan, L., et al. Mol. Cell 37(5):633-642(2010)
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
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