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ELAVL1 Antibody
Mouse Monoclonal Antibody (Mab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IF, WB, E |
|---|---|
| Primary Accession | Q15717 |
| Other Accession | NP_001410.2 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1,K |
| Clone/Animal Names | 224CT6.5.3 |
| Calculated MW | 36092 Da |
| Gene ID | 1994 |
|---|---|
| Other Names | ELAV-like protein 1, Hu-antigen R, HuR, ELAVL1, HUR |
| Target/Specificity | This ELAVL1 monoclonal antibody is generated from mouse immunized with ELAVL1 recombinant protein. |
| Dilution | IF~~1:10~50 WB~~1:1000 |
| Format | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | ELAVL1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ELAVL1 |
|---|---|
| Synonyms | HUR |
| Function | RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability (PubMed:14517288, PubMed:18285462, PubMed:31358969). Involved in embryonic stem cell (ESC) differentiation: preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, promoting ESC differentiation (By similarity). Has also been shown to be capable of binding to m6A-containing mRNAs and contributes to MYC stability by binding to m6A-containing MYC mRNAs (PubMed:32245947). Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs (PubMed:14731398, PubMed:17632515, PubMed:18285462, PubMed:23519412, PubMed:8626503). Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro (PubMed:8626503). With ZNF385A, binds the 3'-UTR of p53/TP53 mRNA to control their nuclear export induced by CDKN2A. Hence, may regulate p53/TP53 expression and mediate in part the CDKN2A anti-proliferative activity. May also bind with ZNF385A the CCNB1 mRNA (By similarity). Increases the stability of the leptin mRNA harboring an AU-rich element (ARE) in its 3' UTR (PubMed:29180010). |
| Cellular Location | Cytoplasm. Nucleus. Cytoplasm, Stress granule {ECO:0000250|UniProtKB:P70372}. Cytoplasm, P-body. Note=Translocates into the cytoplasm following phosphorylation by MAPKAPK2 (PubMed:14517288). Likewise, phosphorylation by PRKCD promotes translocation from the nucleus into the cytoplasm, where it is associated with free and cytoskeleton-bound polysomes (PubMed:18285462). Localizes to the stress granules in the presence of PLEKHN1 (By similarity). {ECO:0000250|UniProtKB:P70372, ECO:0000269|PubMed:14517288, ECO:0000269|PubMed:18285462} |
| Tissue Location | Ubiquitous. Detected in brain, liver, thymus and muscle. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
The protein encoded by this gene is a member of the ELAVL protein family. This encoded protein contains 3 RNA-binding domains and binds cis-acting AU-rich elements. It destabilizes mRNAs and thereby regulates gene expression.
References
Tholanikunnel, B.G., et al. J. Biol. Chem. 285(44):33816-33825(2010)
Drury, G.L., et al. J. Biol. Chem. 285(41):31130-31138(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Raspaglio, G., et al. Cancer Res. 70(14):5891-5900(2010)
Ahn, J., et al. Retrovirology 7, 40 (2010) :
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