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GFAP Antibody
Mouse Monoclonal Antibody (Mab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| IF, WB, IHC-P-Leica, E |
|---|---|
| Primary Accession | P14136 |
| Other Accession | NP_001124491.1 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b,k |
| Clone/Animal Names | 183CT3.1.5 |
| Calculated MW | 49880 Da |
| Gene ID | 2670 |
|---|---|
| Other Names | Glial fibrillary acidic protein, GFAP, GFAP |
| Target/Specificity | This GFAP monoclonal antibody is generated from mouse immunized with GFAP recombinant protein. |
| Dilution | IF~~1:10~50 WB~~1:4000 IHC-P-Leica~~1:1000 IHC~~1:2000 |
| Format | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | GFAP Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | GFAP |
|---|---|
| Function | GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. |
| Cellular Location | Cytoplasm. Note=Associated with intermediate filaments |
| Tissue Location | Expressed in cells lacking fibronectin. |
Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
References
van den Berge, S.A., et al. Aging Cell 9(3):313-326(2010) Martins-de-Souza, D., et al. J Psychiatr Res (2010) In press : Bargagna-Mohan, P., et al. J. Biol. Chem. 285(10):7657-7669(2010) Sultana, R., et al. Antioxid. Redox Signal. 12(3):327-336(2010) Middeldorp, J., et al. PLoS ONE 4 (11), E7663 (2009) :
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