Foundational characteristics of cancer include proliferation, angiogenesis, migration, evasion of apoptosis, and cellular immortality. Find key markers for these cellular processes and antibodies to detect them.
The SUMOplot™ Analysis Program predicts and scores sumoylation sites in your protein. SUMOylation is a post-translational modification involved in various cellular processes, such as nuclear-cytosolic transport, transcriptional regulation, apoptosis, protein stability, response to stress, and progression through the cell cycle.
The Autophagy Receptor Motif Plotter predicts and scores autophagy receptor binding sites in your protein. Identifying proteins connected to this pathway is critical to understanding the role of autophagy in physiological as well as pathological processes such as development, differentiation, neurodegenerative diseases, stress, infection, and cancer.
NGFR Antibody
Purified Mouse Monoclonal Antibody (Mab)
_-_SY5Y.jpg)
- SPECIFICATION
- CITATIONS: 2
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, IF, E |
|---|---|
| Primary Accession | P08138 |
| Reactivity | Human, Mouse |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b,k |
| Clone/Animal Names | 8G3G10 |
| Calculated MW | 45183 Da |
| Gene ID | 4804 |
|---|---|
| Other Names | Tumor necrosis factor receptor superfamily member 16, Gp80-LNGFR, Low affinity neurotrophin receptor p75NTR, Low-affinity nerve growth factor receptor, NGF receptor, p75 ICD, CD271, NGFR, TNFRSF16 |
| Target/Specificity | This NGFR antibody is generated from mouse immunized with NGFR recombinant protein. |
| Dilution | IF~~1:100 WB~~1:500~1000 IHC-P~~1:50~100 |
| Format | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | NGFR Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | NGFR |
|---|---|
| Synonyms | TNFRSF16 |
| Function | Low affinity receptor which can bind to NGF, BDNF, NTF3, and NTF4. Forms a heterodimeric receptor with SORCS2 that binds the precursor forms of NGF, BDNF and NTF3 with high affinity, and has much lower affinity for mature NGF and BDNF (PubMed:24908487). Plays an important role in differentiation and survival of specific neuronal populations during development (By similarity). Can mediate cell survival as well as cell death of neural cells. Plays a role in the inactivation of RHOA (PubMed:26646181). Plays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin, probably by regulating RAB31 activity, and thereby contributes to the regulation of insulin- dependent glucose uptake (By similarity). Necessary for the circadian oscillation of the clock genes BMAL1, PER1, PER2 and NR1D1 in the suprachiasmatic nucleus (SCmgetaN) of the brain and in liver and of the genes involved in glucose and lipid metabolism in the liver (PubMed:23785138). |
| Cellular Location | Cell membrane; Single-pass type I membrane protein. Perikaryon {ECO:0000250|UniProtKB:Q9Z0W1}. Cell projection, growth cone {ECO:0000250|UniProtKB:Q9Z0W1}. Cell projection, dendritic spine {ECO:0000250|UniProtKB:Q9Z0W1} |
Provided below are standard protocols that you may find useful for product applications.
Background
Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain.
References
References for protein:
1. Human melanoma-initiating cells express neural crest nerve growth factor receptor CD271. Boiko AD, et al. Nature, 2010 Jul 1. PMID 20596026. Interleukin-9 polymorphism in infants with respiratory syncytial virus infection: an opposite effect in boys and girls. Schuurhof A, et al. Pediatr Pulmonol, 2010 Jun. PMID:20503287.
2. Poor replication of candidate genes for major depressive disorder using genome-wide association data. Bosker FJ, et al. Mol Psychiatry, 2010 Mar 30. PMID 20351714.
3. New genetic associations detected in a host response study to hepatitis B vaccine. Davila S, et al. Genes Immun, 2010 Apr. PMID 20237496.
4. Role of the neurotrophin network in eating disorders' subphenotypes: body mass index and age at onset of the disease. Gratacòs M, et al. J Psychiatr Res, 2010 Oct. PMID 20219210.
References for SY5Y (SH-SY5Y; ATCC#CRL-2266): 1. Ross RA, et al. Coordinate morphological and biochemical interconversion of human neuroblastoma cells. J. Natl. Cancer Inst. 71: 741-749, 1983. [PubMed: 6137586]; 2. Biedler JL, et al. Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res. 38: 3751-3757, 1978. [PubMed: 29704].
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.
Ordering Information
Other Products
Shipping Information
