CD63 Antibody (clone AHN16.1/46-4-5)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| IHC-P, ICC, FC |
---|---|
Primary Accession | P08962 |
Other Accession | 967 |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Isotype | IgG1 |
Clone Names | AHN16.1/46-4-5 |
Calculated MW | 25637 Da |
Dilution | IHC-P (10 µg/ml), |
Gene ID | 967 |
---|---|
Other Names | CD63, CD63 molecule, LAMP-3, ME491, Granulophysin, MLA1, Tetraspanin-30, Tspan-30, CD63 antigen, OMA81H, TSPAN30 |
Target/Specificity | CD63 molecule of about 50 kd. |
Reconstitution & Storage | 50 mM sodium phosphate, pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 0.5 mg/ml Gentamicin sulfate. Store at +4°C. DO NOT FREEZE. |
Precautions | CD63 Antibody (clone AHN16.1/46-4-5) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | CD63 |
---|---|
Synonyms | MLA1, TSPAN30 |
Function | Functions as a cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli. |
Cellular Location | Cell membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Endosome, multivesicular body. Melanosome. Secreted, extracellular exosome. Cell surface. Note=Also found in Weibel-Palade bodies of endothelial cells (PubMed:10793155). Located in platelet dense granules (PubMed:7682577). Detected in a subset of pre-melanosomes Detected on intralumenal vesicles (ILVs) within multivesicular bodies (PubMed:21962903). |
Tissue Location | Detected in platelets (at protein level). Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages. |
Volume | 50 µl |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli.
References
Hotta H.,et al.Cancer Res. 48:2955-2962(1988).
Rapp G.,et al.DNA Cell Biol. 9:479-485(1990).
Metzelaar M.J.,et al.J. Biol. Chem. 266:3239-3245(1991).
Wang M.X.,et al.Arch. Ophthalmol. 110:399-404(1992).
Hotta H.,et al.Biochem. Biophys. Res. Commun. 185:436-442(1992).
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.