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PGK1/Phosphoglycerate Kinase 1 Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | P00558 |
| Other Accession | 5230 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 44615 Da |
| Dilution | IHC-P (10 µg/ml), |
| Gene ID | 5230 |
|---|---|
| Other Names | PGK1, MIG10, Primer recognition protein 2, PRP 2, PGKA, Phosphoglycerate kinase 1 |
| Target/Specificity | Human PGK1 |
| Reconstitution & Storage | PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol. Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles. |
| Precautions | PGK1/Phosphoglycerate Kinase 1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PGK1 |
|---|---|
| Synonyms | PGKA |
| Function | Catalyzes one of the two ATP producing reactions in the glycolytic pathway via the reversible conversion of 1,3- diphosphoglycerate to 3-phosphoglycerate (PubMed:30323285, PubMed:7391028). Both L- and D- forms of purine and pyrimidine nucleotides can be used as substrates, but the activity is much lower on pyrimidines (PubMed:18463139). In addition to its role as a glycolytic enzyme, it seems that PGK1 acts as a polymerase alpha cofactor protein (primer recognition protein) (PubMed:2324090). Acts as a protein kinase when localized to the mitochondrion where it phosphorylates pyruvate dehydrogenase kinase PDK1 to inhibit pyruvate dehydrogenase complex activity and suppress the formation of acetyl- coenzyme A from pyruvate, and consequently inhibit oxidative phosphorylation and promote glycolysis (PubMed:26942675, PubMed:36849569). May play a role in sperm motility (PubMed:26677959). |
| Cellular Location | Cytoplasm, cytosol. Mitochondrion matrix. Note=Hypoxic conditions promote mitochondrial targeting (PubMed:26942675). Targeted to the mitochondrion following phosphorylation by MAPK1/ERK2, cis-trans isomerization by PIN1, and binding to mitochondrial circRNA mcPGK1 (PubMed:36849569). |
| Tissue Location | Mainly expressed in spermatogonia. Localized on the principle piece in the sperm (at protein level). Expression significantly decreased in the testis of elderly men |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein).
References
Michelson A.M.,et al.Proc. Natl. Acad. Sci. U.S.A. 80:472-476(1983).
Michelson A.M.,et al.Proc. Natl. Acad. Sci. U.S.A. 82:6965-6969(1985).
Kim J.W.,et al.Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases.
Shichijo S.,et al.Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
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