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SPP1 / Osteopontin Antibody (N-Terminus)

Rabbit Polyclonal Antibody

     
  • IHC - SPP1 / Osteopontin Antibody (N-Terminus) ALS16768
    Anti-SPP1 / Osteopontin antibody IHC staining of human kidney.
    detail
  • IF - SPP1 / Osteopontin Antibody (N-Terminus) ALS16768
    Immunofluorescence of SPP1 in human bladder tissue with SPP1 antibody at 20 ug/ml.
    detail
  • WB - SPP1 / Osteopontin Antibody (N-Terminus) ALS16768
    Western blot analysis of SPP1 in human bladder tissue lysate with SPP1 antibody at (A) 1 and (B)...
    detail
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession P10451
Other Accession 6696
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 35423 Da
Dilution IF (20 µg/ml), IHC-P (10 µg/ml), WB (1 - 2 µg/ml),
Additional Information
Gene ID 6696
Other Names SPP1, BSPI, BNSP, Bone sialoprotein 1, ETA-1, Nephropontin, OPN, Secreted phosphoprotein 1, SPP-1, SPP1/CALPHA1 fusion, Urinary stone protein, Uropontin, Osteopontin, Bone sialoprotein I
Target/Specificity SPP1 antibody is human, mouse and rat reactive. Multiple isoforms of SPP1 are known to exist.
Reconstitution & Storage PBS, 0.02% sodium azide. Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
PrecautionsSPP1 / Osteopontin Antibody (N-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SPP1
Synonyms BNSP, OPN
Function Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Cellular Location Secreted
Tissue Location Detected in cerebrospinal fluid and urine (at protein level) (PubMed:25326458, PubMed:36213313, PubMed:37453717) Bone. Found in plasma.
Research Areas
Citations (0)
citation

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Background

Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.

References

Kiefer M.C.,et al.Nucleic Acids Res. 17:3306-3306(1989).
Young M.F.,et al.Genomics 7:491-502(1990).
Shiraga H.,et al.Proc. Natl. Acad. Sci. U.S.A. 89:426-430(1992).
Crosby A.H.,et al.Genomics 27:155-160(1995).
Hijiya N.,et al.Biochem. J. 303:255-262(1994).

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$ 467.50
Cat# ALS16768
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