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QTRT1 Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P |
|---|---|
| Primary Accession | Q9BXR0 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 44kDa |
| Dilution | IHC-P (5 µg/ml), WB (1:500-1:3000), |
| Gene ID | 81890 |
|---|---|
| Other Names | Queuine tRNA-ribosyltransferase, 2.4.2.29, Guanine insertion enzyme, tRNA-guanine transglycosylase, QTRT1, TGT, TGUT |
| Target/Specificity | Human, mouse QTRT1 |
| Reconstitution & Storage | Aliquot and freeze at -20° C. Avoid freeze-thaw cycles. |
| Precautions | QTRT1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | QTRT1 {ECO:0000255|HAMAP-Rule:MF_03218} |
|---|---|
| Synonyms | TGT, TGUT |
| Function | Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2- cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) (PubMed:11255023, PubMed:20354154, PubMed:34009357, PubMed:34241577). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product (By similarity). Modification of cytoplasmic tRNAs with queuosine controls the elongation speed of cognate codons, thereby ensuring the correct folding of nascent proteins to maintain proteome integrity (PubMed:30093495). |
| Cellular Location | Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03218}. Mitochondrion outer membrane {ECO:0000255|HAMAP-Rule:MF_03218}; Peripheral membrane protein {ECO:0000255|HAMAP-Rule:MF_03218}; Cytoplasmic side {ECO:0000255|HAMAP-Rule:MF_03218}. Note=Weakly associates with mitochondria, possibly via QTRT2. {ECO:0000255|HAMAP- Rule:MF_03218} |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Interacts with QTRTD1 to form an active queuine tRNA- ribosyltransferase. This enzyme exchanges queuine for the guanine at the wobble position of tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), thereby forming the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1- yl)amino)methyl)-7-deazaguanosine) (By similarity).
References
Deshpande K.L.,et al.Gene 265:205-212(2001).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Grimwood J.,et al.Nature 428:529-535(2004).
Burkard T.R.,et al.BMC Syst. Biol. 5:17-17(2011).
Van Damme P.,et al.Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
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