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CANX / Calnexin Antibody (C-Terminus)
Goat Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IF |
|---|---|
| Primary Accession | P27824 |
| Reactivity | Human, Mouse, Rat, Monkey, Dog |
| Host | Goat |
| Clonality | Polyclonal |
| Calculated MW | 68kDa |
| Dilution | IF (1:50-1:500), IHC-P (5 µg/ml), WB (1:500-1:2000) |
| Gene ID | 821 |
|---|---|
| Other Names | Calnexin, IP90, Major histocompatibility complex class I antigen-binding protein p88, p90, CANX |
| Target/Specificity | Detects a band of 90 kDa by Western blot in the following human (293A, primary fibroblasts, HaCat, HeLa, HMEC-1, Jurkat, MNT1, U-118, rat (TR-iBRB), mouse (3T3, AtT-20, Hepa, Raw264.7), monkey (COS-7) and canine (D17) whole cell lysates. |
| Reconstitution & Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
| Precautions | CANX / Calnexin Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CANX |
|---|---|
| Function | Calcium-binding protein that interacts with newly synthesized monoglucosylated glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse. |
| Cellular Location | Endoplasmic reticulum membrane; Single-pass type I membrane protein. Mitochondrion membrane {ECO:0000250|UniProtKB:P24643}; Single-pass type I membrane protein. Melanosome membrane; Single-pass type I membrane protein. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:12643545, PubMed:17081065). The palmitoylated form preferentially localizes to the perinuclear rough ER (PubMed:22314232) Localizes to endoplasmic reticulum mitochondria-associated membrane (MAMs) that connect the endoplasmic reticulum and the mitochondria (By similarity). {ECO:0000250|UniProtKB:P24643, ECO:0000269|PubMed:12643545, ECO:0000269|PubMed:17081065, ECO:0000269|PubMed:22314232} |
| Volume | 100 µl |
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Provided below are standard protocols that you may find useful for product applications.
Background
Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor- mediated endocytosis at the synapse.
References
David V.,et al.J. Biol. Chem. 268:9585-9592(1993).
Tjoelker L.W.,et al.Biochemistry 33:3229-3236(1994).
Honore B.,et al.Electrophoresis 15:482-490(1994).
Hansen J.J.,et al.Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
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