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NR3C1/Glucocorticoid Receptor Antibody (clone 6E6)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IF, ICC, E, FC |
|---|---|
| Primary Accession | P04150 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | 6E6 |
| Calculated MW | 86kDa |
| Dilution | ELISA (1:10000), Flo (1:200-1:400), ICC (1:200-1:1000), IF (1:1000), IHC-P (1:200), WB (1:500-1:2000), |
| Gene ID | 2908 |
|---|---|
| Other Names | Glucocorticoid receptor, GR, Nuclear receptor subfamily 3 group C member 1, NR3C1, GRL |
| Target/Specificity | Human GR / Glucocorticoid Receptor |
| Reconstitution & Storage | Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles. |
| Precautions | NR3C1/Glucocorticoid Receptor Antibody (clone 6E6) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | NR3C1 (HGNC:7978) |
|---|---|
| Synonyms | GRL |
| Function | Receptor for glucocorticoids (GC) (PubMed:27120390, PubMed:37478846). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors (PubMed:28139699). Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Involved in chromatin remodeling (PubMed:9590696). Plays a role in rapid mRNA degradation by binding to the 5' UTR of target mRNAs and interacting with PNRC2 in a ligand-dependent manner which recruits the RNA helicase UPF1 and the mRNA-decapping enzyme DCP1A, leading to RNA decay (PubMed:25775514). Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth (By similarity). |
| Cellular Location | [Isoform Alpha]: Cytoplasm. Nucleus. Mitochondrion. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=After ligand activation, translocates from the cytoplasm to the nucleus. In the presence of NR1D1 shows a time-dependent subcellular localization, localizing to the cytoplasm at ZT8 and to the nucleus at ZT20 (By similarity). Lacks this diurnal pattern of localization in the absence of NR1D1, localizing to both nucleus and the cytoplasm at ZT8 and ZT20 (By similarity). {ECO:0000250|UniProtKB:P06537, ECO:0000269|PubMed:18838540, ECO:0000269|PubMed:27120390, ECO:0000269|PubMed:8621628} [Isoform Alpha-B]: Nucleus. Cytoplasm Note=After ligand activation, translocates from the cytoplasm to the nucleus. |
| Tissue Location | Widely expressed including bone, stomach, lung, liver, colon, breast, ovary, pancreas and kidney (PubMed:25847991). In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart (PubMed:10902803) [Isoform Alpha-2]: Widely expressed. |
| Volume | 50 µl |
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Provided below are standard protocols that you may find useful for product applications.
Background
Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. May play a negative role in adipogenesis through the regulation of lipolytic and antilipogenic genes expression.
References
Hollenberg S.M.,et al.Nature 318:635-641(1985).
Encio I.J.,et al.J. Biol. Chem. 266:7182-7188(1991).
Wang W.,et al.Nucleic Acids Res. 39:44-58(2011).
Turner J.D.,et al.Ann. N. Y. Acad. Sci. 1095:334-341(2007).
Munroe D.G.,et al.Submitted (SEP-1993) to the EMBL/GenBank/DDBJ databases.
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