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PLA2G4A Antibody (aa471-520)

Rabbit Polyclonal Antibody

     
  • IF - PLA2G4A Antibody (aa471-520) ALS15006
    Immunofluorescence of HUVEC cells, using c-PLA2 Antibody.
    detail
  • WB - PLA2G4A Antibody (aa471-520) ALS15006
    Western blot of extracts from HeLa cells, treated with TNF-a 20 ng/ml 30', using c-PLA2 Antibody.
    detail
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession P47712
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 85kDa
Dilution ELISA (1:10000), IF (1:100-1:500), IHC-P (10 µg/ml), WB (1:500-1:1000) ,
Additional Information
Gene ID 5321
Other Names Cytosolic phospholipase A2, cPLA2, Phospholipase A2 group IVA, Phospholipase A2, 3.1.1.4, Phosphatidylcholine 2-acylhydrolase, Lysophospholipase, 3.1.1.5, PLA2G4A, CPLA2, PLA2G4
Target/Specificity c-PLA2 Antibody detects endogenous levels of total c-PLA2 protein.
Reconstitution & Storage Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
PrecautionsPLA2G4A Antibody (aa471-520) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PLA2G4A
Synonyms CPLA2, PLA2G4
Function Has primarily calcium-dependent phospholipase and lysophospholipase activities, with a major role in membrane lipid remodeling and biosynthesis of lipid mediators of the inflammatory response (PubMed:10358058, PubMed:14709560, PubMed:16617059, PubMed:17472963, PubMed:18451993, PubMed:27642067, PubMed:7794891, PubMed:8619991, PubMed:8702602, PubMed:9425121). Plays an important role in embryo implantation and parturition through its ability to trigger prostanoid production (By similarity). Preferentially hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity) (PubMed:10358058, PubMed:17472963, PubMed:18451993, PubMed:7794891, PubMed:8619991, PubMed:9425121). Selectively hydrolyzes sn-2 arachidonoyl group from membrane phospholipids, providing the precursor for eicosanoid biosynthesis via the cyclooxygenase pathway (PubMed:10358058, PubMed:17472963, PubMed:18451993, PubMed:7794891, PubMed:9425121). In an alternative pathway of eicosanoid biosynthesis, hydrolyzes sn-2 fatty acyl chain of eicosanoid lysophopholipids to release free bioactive eicosanoids (PubMed:27642067). Hydrolyzes the ester bond of the fatty acyl group attached at sn-1 position of phospholipids (phospholipase A1 activity) only if an ether linkage rather than an ester linkage is present at the sn-2 position. This hydrolysis is not stereospecific (PubMed:7794891). Has calcium-independent phospholipase A2 and lysophospholipase activities in the presence of phosphoinositides (PubMed:12672805). Has O-acyltransferase activity. Catalyzes the transfer of fatty acyl chains from phospholipids to a primary hydroxyl group of glycerol (sn-1 or sn-3), potentially contributing to monoacylglycerol synthesis (PubMed:7794891).
Cellular Location Cytoplasm. Golgi apparatus membrane. Nucleus envelope Note=Translocates to intracellular membranes in a calcium-dependent way.
Tissue Location Expressed in various cells and tissues such as macrophages, neutrophils, fibroblasts and lung endothelium. Expressed in platelets (at protein level) (PubMed:25102815)
Volume 50 µl
Research Areas
Citations (0)
citation

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Background

Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response.

References

Clark J.D.,et al.Cell 65:1043-1051(1991).
Sharp J.,et al.J. Biol. Chem. 266:14850-14853(1991).
Gregory S.G.,et al.Nature 441:315-321(2006).
Lin L.-L.,et al.Cell 72:269-278(1993).
Sharp J.D.,et al.J. Biol. Chem. 269:23250-23254(1994).

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$ 467.50
Cat# ALS15006
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