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ATP1A1 Antibody (aa5-54)

Rabbit Polyclonal Antibody

     
  • IF - ATP1A1 Antibody (aa5-54) ALS14721
    Immunofluorescence of COS7 cells, using ATPase antibody.
    detail
  • WB - ATP1A1 Antibody (aa5-54) ALS14721
    Western blot of extracts from 293 cells, treated with PMA 125 ng/ml 30', using ATPase antibody.
    detail
  • IHC - ATP1A1 Antibody (aa5-54) ALS14721
    Anti-ATP1A1 antibody IHC of human pancreas.
    detail
  • SPECIFICATION
  • CITATIONS
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  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession P05023
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 113kDa
Dilution ELISA (1:5000), IF (1:100-1:500), IHC-P (1:50-1:100), WB (1:500-1:1000) ,
Additional Information
Gene ID 476
Other Names Sodium/potassium-transporting ATPase subunit alpha-1, Na(+)/K(+) ATPase alpha-1 subunit, 3.6.3.9, Sodium pump subunit alpha-1, ATP1A1
Target/Specificity ATPase antibody detects endogenous levels of total ATPase protein.
Reconstitution & Storage Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
PrecautionsATP1A1 Antibody (aa5-54) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ATP1A1
Function This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients (PubMed:29499166, PubMed:30388404). Could also be part of an osmosensory signaling pathway that senses body-fluid sodium levels and controls salt intake behavior as well as voluntary water intake to regulate sodium homeostasis (By similarity).
Cellular Location Cell membrane {ECO:0000250|UniProtKB:Q8VDN2}; Multi-pass membrane protein. Basolateral cell membrane {ECO:0000250|UniProtKB:P06685}; Multi-pass membrane protein. Cell membrane, sarcolemma; Multi-pass membrane protein. Cell projection, axon {ECO:0000250|UniProtKB:P06685}. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV
Volume 50 µl
Research Areas
Citations (0)
citation

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Background

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.

References

Kawakami K.,et al.J. Biochem. 100:389-397(1986).
Ruiz A.,et al.Gene 155:179-184(1995).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Gregory S.G.,et al.Nature 441:315-321(2006).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

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$ 467.50
Cat# ALS14721
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