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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Anti-Wnt-Pathway (plus Lgr5) Antibodies   >   TNF Alpha Antibody (clone M 1 C4)   

TNF Alpha Antibody (clone M 1 C4)

Mouse Monoclonal Antibody

     
  • WB - TNF Alpha Antibody (clone M 1 C4) ALS14356
    TNF monoclonal antibody, ALS14356. Western blot of TNF expression in rat liver.
    detail
  • IHC - TNF Alpha Antibody (clone M 1 C4) ALS14356
    Anti-TNF Alpha antibody IHC of human skin.
    detail
  • IHC - TNF Alpha Antibody (clone M 1 C4) ALS14356
    Anti-TNF Alpha antibody IHC of human brain, cerebellum.
    detail
  • SPECIFICATION
  • CITATIONS: 1
  • PROTOCOLS
  • BACKGROUND
  • detail
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P01375
Reactivity Human, Rat
Host Mouse
Clonality Monoclonal
Clone Names M 1 C4
Calculated MW 26kDa
Dilution IHC-P (5 µg/ml), WB (1:500-1:1000),
Additional Information
Gene ID 7124
Other Names Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Tumor necrosis factor, membrane form, N-terminal fragment, NTF, Intracellular domain 1, ICD1, Intracellular domain 2, ICD2, C-domain 1, C-domain 2, Tumor necrosis factor, soluble form, TNF, TNFA, TNFSF2
Target/Specificity Human TNF
Reconstitution & Storage Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
PrecautionsTNF Alpha Antibody (clone M 1 C4) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TNF
Synonyms TNFA, TNFSF2
Function Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T- cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Up-regulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line (PubMed:16829952, PubMed:22517918, PubMed:23396208). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (By similarity). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (PubMed:12794819). Promotes osteoclastogenesis and therefore mediates bone resorption (By similarity).
Cellular Location Cell membrane; Single-pass type II membrane protein [Tumor necrosis factor, soluble form]: Secreted [C-domain 2]: Secreted.
Research Areas
Citations ( 0 )

Background

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.

References

Nedospasov S.A.,et al.Cold Spring Harb. Symp. Quant. Biol. 51:611-624(1986).
Pennica D.,et al.Nature 312:724-729(1984).
Shirai T.,et al.Nature 313:803-806(1985).
Nedwin G.E.,et al.Nucleic Acids Res. 13:6361-6373(1985).
Wang A.M.,et al.Science 228:149-154(1985).

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$ 533.50
Cat# ALS14356
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