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ACTB / Beta Actin Antibody (clone 1E7)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | P60709 |
| Reactivity | Human, Mouse, Rat |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | 1E7 |
| Calculated MW | 42kDa |
| Dilution | IHC-P (5 µg/ml), WB (1:500-1:1000), |
| Gene ID | 60 |
|---|---|
| Other Names | Actin, cytoplasmic 1, Beta-actin, Actin, cytoplasmic 1, N-terminally processed, ACTB |
| Target/Specificity | Human Beta Actin |
| Reconstitution & Storage | Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles. |
| Precautions | ACTB / Beta Actin Antibody (clone 1E7) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ACTB |
|---|---|
| Function | Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed:25255767, PubMed:29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed:29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F- actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed:29925947). Part of the ACTR1A/ACTB filament around which the dynactin complex is built. The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity). |
| Cellular Location | Cytoplasm, cytoskeleton. Nucleus Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
References
Ponte P.,et al.Nucleic Acids Res. 12:1687-1696(1984).
Nakajima-Iijima S.,et al.Proc. Natl. Acad. Sci. U.S.A. 82:6133-6137(1985).
Ohmori H.,et al.J. Cell Biol. 116:933-941(1992).
Hillier L.W.,et al.Nature 424:157-164(2003).
Gevaert K.,et al.Nat. Biotechnol. 21:566-569(2003).
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