DGAT1 Antibody (Internal)
Goat Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, E |
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Primary Accession | O75907 |
Reactivity | Human, Mouse, Rat, Hamster, Monkey, Pig, Goat, Sheep, Horse, Bovine, Dog |
Host | Goat |
Clonality | Polyclonal |
Calculated MW | 55kDa |
Dilution | ELISA (1:64000), IHC-P (3.75 µg/ml), WB (0.3-1 µg/ml) |
Gene ID | 8694 |
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Other Names | Diacylglycerol O-acyltransferase 1, 2.3.1.20, ACAT-related gene product 1, Acyl-CoA retinol O-fatty-acyltransferase, ARAT, Retinol O-fatty-acyltransferase, 2.3.1.76, Diglyceride acyltransferase, DGAT1, AGRP1, DGAT |
Target/Specificity | Human DGAT1. |
Reconstitution & Storage | Store at -20°C. Minimize freezing and thawing. |
Precautions | DGAT1 Antibody (Internal) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | DGAT1 {ECO:0000303|PubMed:16214399, ECO:0000312|HGNC:HGNC:2843} |
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Function | Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates (PubMed:16214399, PubMed:18768481, PubMed:28420705, PubMed:32433610, PubMed:32433611, PubMed:9756920). Highly expressed in epithelial cells of the small intestine and its activity is essential for the absorption of dietary fats (PubMed:18768481). In liver, plays a role in esterifying exogenous fatty acids to glycerol, and is required to synthesize fat for storage (PubMed:16214399). Also present in female mammary glands, where it produces fat in the milk (By similarity). May be involved in VLDL (very low density lipoprotein) assembly (PubMed:18768481). In contrast to DGAT2 it is not essential for survival (By similarity). Functions as the major acyl-CoA retinol acyltransferase (ARAT) in the skin, where it acts to maintain retinoid homeostasis and prevent retinoid toxicity leading to skin and hair disorders (PubMed:16214399). Exhibits additional acyltransferase activities, includin acyl CoA:monoacylglycerol acyltransferase (MGAT), wax monoester and wax diester synthases (By similarity). Also able to use 1-monoalkylglycerol (1-MAkG) as an acyl acceptor for the synthesis of monoalkyl-monoacylglycerol (MAMAG) (PubMed:28420705). |
Cellular Location | Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q9Z2A7}; Multi-pass membrane protein |
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Background
Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. In contrast to DGAT2 it is not essential for survival. May be involved in VLDL (very low density lipoprotein) assembly. In liver, plays a role in esterifying exogenous fatty acids to glycerol. Functions as the major acyl-CoA retinol acyltransferase (ARAT) in the skin, where it acts to maintain retinoid homeostasis and prevent retinoid toxicity leading to skin and hair disorders.
References
Oelkers P.,et al.J. Biol. Chem. 273:26765-26771(1998).
Yamasaki Y.,et al.Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases.
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Orland M.D.,et al.Biochim. Biophys. Acta 1737:76-82(2005).
Haas J.T.,et al.J. Clin. Invest. 122:4680-4684(2012).
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