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DES / Desmin Antibody (clone D33)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IHC-F |
|---|---|
| Primary Accession | P17661 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | D33 |
| Calculated MW | 54kDa |
| Dilution | IHC-P (1:100) |
| Gene ID | 1674 |
|---|---|
| Other Names | Desmin, DES |
| Target/Specificity | This antibody is highly reactive with desmin. On immunoblots only the 52 kD desmin band is stained. Immunogen derived from human Leiomyoma. |
| Reconstitution & Storage | Store at 4°C. |
| Precautions | DES / Desmin Antibody (clone D33) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | DES |
|---|---|
| Function | Muscle-specific type III intermediate filament essential for proper muscular structure and function. Plays a crucial role in maintaining the structure of sarcomeres, inter-connecting the Z-disks and forming the myofibrils, linking them not only to the sarcolemmal cytoskeleton, but also to the nucleus and mitochondria, thus providing strength for the muscle fiber during activity (PubMed:25358400). In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z- line structures (PubMed:24200904, PubMed:25394388, PubMed:26724190). May act as a sarcomeric microtubule-anchoring protein: specifically associates with detyrosinated tubulin-alpha chains, leading to buckled microtubules and mechanical resistance to contraction. Required for nuclear membrane integrity, via anchoring at the cell tip and nuclear envelope, resulting in maintenance of microtubule-derived intracellular mechanical forces (By similarity). Contributes to the transcriptional regulation of the NKX2-5 gene in cardiac progenitor cells during a short period of cardiomyogenesis and in cardiac side population stem cells in the adult. Plays a role in maintaining an optimal conformation of nebulette (NEB) on heart muscle sarcomeres to bind and recruit cardiac alpha-actin (By similarity). |
| Cellular Location | Cytoplasm, myofibril, sarcomere, Z line. Cytoplasm Cell membrane, sarcolemma. Nucleus {ECO:0000250|UniProtKB:P31001}. Cell tip {ECO:0000250|UniProtKB:P31001}. Nucleus envelope {ECO:0000250|UniProtKB:P31001}. Note=Localizes in the intercalated disks which occur at the Z line of cardiomyocytes (PubMed:24200904, PubMed:26724190). Localizes in the nucleus exclusively in differentiating cardiac progenitor cells and premature cardiomyocytes (By similarity). PKP2 is required for correct anchoring of DES at the cell tip and nuclear envelope (By similarity) {ECO:0000250|UniProtKB:P31001, ECO:0000269|PubMed:24200904, ECO:0000269|PubMed:26724190} |
| Volume | 100 µl |
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Provided below are standard protocols that you may find useful for product applications.
Background
Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures.
References
Li Z.,et al.Gene 78:243-254(1989).
Li Z.,et al.J. Biol. Chem. 266:6562-6570(1991).
Vicart P.,et al.Hum. Genet. 98:422-429(1996).
Goldfarb L.G.,et al.Nat. Genet. 19:402-403(1998).
Li D.,et al.Circulation 100:461-464(1999).
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